鸡β-防御素基因的克隆、序列分析及其在组织中的分布

畜牧兽医学报 ›› 2008, Vol. 39 ›› Issue (8): 1033-1039.doi:

鸡β-防御素基因的克隆、序列分析及其在组织中的分布

马得莹¹*，刘胜旺²，李一经³，单安山¹

1.东北农业大学动物科学技术学院动物营养研究所，哈尔滨 150030；2.中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室禽病室, 哈尔滨 150001；3.东北农业大学动物医学院，哈尔滨 150030

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-08-24 发布日期:2008-08-24
通讯作者: 马得莹

Cloning, Sequencing and Distribution of Chick β-defensins Genes

MA De-ying¹*，LIU Sheng-wang², LI Yi-jing³，SHAN An-shan¹

1.Institute of Animal Nutrition, Department of Animal Science and Technology,Northeast Agricultural University, Harbin 150030,China;2.Division of Avian Infectious Disease, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001 ,China;3.College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030,China

Received:1900-01-01 Revised:1900-01-01 Online:2008-08-24 Published:2008-08-24
Contact: MA De-ying

摘要/Abstract

摘要： 本研究采用RT-PCR法，分别从鸡肝脏和舌组织中扩增到鸡β-防御素（Gallinacin， Gal）Gal-6、Gal-8与Gal-9基因，经序列测定表明，Gal-6、Gal-8和Gal-9大小分别为201、204和201 bp，其成熟肽分别由67、68和67个氨基酸残基组成。组织表达分析表明，Gal-6基因在鸡体内各组织器官中广泛地表达，在鸡皮肤、舌、心脏、小肠、胸肌、肝脏、肾脏、法氏囊、脾脏和胰腺中表达量较高；在肺脏、睾丸和骨髓中也有一定量表达。相比之下，Gal-8在鸡体内的表达较为局限，Gal-8基因仅在舌、小肠、肺脏和肝脏中大量表达。Gal-9基因在鸡体内组织中分布也较广泛，在舌和小肠中大量表达，在气管、肺脏、肝脏、肾脏、骨髓和胸腺有少量表达。

Abstract: Three Gallinacin (Gal) genes: Gal-6, Gal-8 and Gal-9 were cloned from liver and tongue of chicks by RT-PCR, respectively. In addition, we have sequenced these genes. The full cDNA length of Gal-6 and Gal-9 consist of 201 bp encoding 67 amino acids, while Gal-8 consist of 204 bp encoding 68 amino acids. Differential gene expression of Gal-6, Gal-9 and Gal-8 has been demonstrated across a panel of tissues in the chick. It has been shown that Gal-6 mRNA was highly expressed in the skin, tongue, liver, heart, small intestine, breast muscle, kidney, bursa of Fabricius, spleen, and pancreas, and moderately expressed in the lung, testis, and borrow marrow. Gal-8 mRNA was highly expressed in the liver, tongue, small intestine and lung of chick. While, Gal-9 mRNA was highly expressed in the tongue and small intestine, and moderately expressed in the trachea, lung, liver, kidney, borrow marrow and thymus of chick.

马得莹;刘胜旺;李一经;单安山. 鸡β-防御素基因的克隆、序列分析及其在组织中的分布[J]. 畜牧兽医学报, 2008, 39(8): 1033-1039.

MA De-ying;LIU Sheng-wang;LI Yi-jing;SHAN An-shan. Cloning, Sequencing and Distribution of Chick β-defensins Genes[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2008, 39(8): 1033-1039.

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