畜牧兽医学报 ›› 2008, Vol. 39 ›› Issue (7): 994-999.doi:

• 研究简报 • 上一篇    下一篇

鹅固醇调节元件结合蛋白1基因的克隆及生物信息学分析

韩春春, 王继文*   

  1. 四川农业大学畜禽品种资源发掘与利用重点实验室,雅安 625014
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-07-23 发布日期:2008-07-23
  • 通讯作者: 王继文

DNA Segment Cloning and Bioinformatics Analysis of SREBP-1 Gene in the Landes Goose

HAN Chun-chun, WANG Ji-wen*   

  1. Key Lab of Animal Genetic Resources, Sichuan Agricultural University, Ya’an 625014,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-07-23 Published:2008-07-23

摘要: 从16周龄朗德鹅肝脏组织提取总RNA,采用反转录-多聚酶链式反应(RT-PCR)方法扩增出1条长约280 bp的cDNA片段。序列分析结果表明,扩增出的cDNA片段与固醇调节元件结合蛋白1(SREBP-1)基因有较高的同源性,并且该片段包含着1个bHLH功能区。同时该片段包含1个明显的亲水区和1个明显的疏水区,二级结构富含α-螺旋,这与其功能相符。

Abstract: Total RNA from Landes goose liver of 16 weeks old was extracted.The cDNA segment about 282 bp of Langdes goose was amplified by the reverse transcription-polymerase chain reaction(RT-PCR)Sequence analysis showed that there is a high similarity between the fragment of cDNA and sterol regulatory element binding protein-1(SREBP-1) gene containing a bHLH function region. Meanwhile, the segment evidently has a hydrophilic region and a hydrophobic region, and the secondary structure richly contains αhelix, which accords with its function.