畜牧兽医学报 ›› 2008, Vol. 39 ›› Issue (7): 873-878.doi:

• 遗传繁育 • 上一篇    下一篇

皖西白鹅BMP4基因部分cDNA序列的克隆及序列分析

郭炫,吴伟*,高光,孙永峰,隋玉健   

  1. 吉林农业大学动物科技学院,长春 130118
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-07-23 发布日期:2008-07-23
  • 通讯作者: 吴伟

Cloning and Sequence Analysis of a Partial cDNA Encoding BMP4 of Wanxi White Goose

GUO Xuan, WU Wei*, GAO Guang, SUN Yong-feng, SUI Yu-jian   

  1. College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-07-23 Published:2008-07-23

摘要: 根据GenBank中鸡BMP4基因序列设计1对特异性引物,从12胚龄皖西白鹅背部皮肤中提取总RNA作为模板,采用RT-PCR技术首次扩增出鹅BMP4基因的部分cDNA序列。将其克隆进pGEM-T easy vector中,经限制酶酶切鉴定后进行测序。将测序结果与鸡、大鼠、小鼠、人和黑猩猩的BMP4核苷酸序列进行比对,发现多处突变。转换成氨基酸序列后发现鹅BMP4的氨基酸序列与其他物种也有多处不同。分析这些突变可能是导致禽类羽毛和哺乳动物毛发之间形态学差异的原因之一。

Abstract: According to the chicken BMP4 gene sequence in GenBank, a pair of specific primers were designed, and total RNA extracted from the dorsal skin of Wanxi White goose at embryonic day 12 was used to amplify a partial cDNA encoding BMP4 with RT-PCR technique. The PCR product was cloned into pGEM-T easy vector, and the recombinant plasmid was digested with restriction endonuclease, and then the positive plasmid was sent to be sequenced. After being aligned with the related cDNA sequences of Gallus gallus, Rattus norvegicus, Mus musculus, Homo sapiens and Pan troglodytes, some mutations were found. When being translated into amino acid sequence, some mutations in BMP4 among goose and other species were also observed. These mutations may cause the differentiation in the morphology between the feathers of the fowl and the hairs of the mammal.