Abstract: The LIPI-1 gene cluster of Listeria monocytogenes isolate strain XFL0605 was segmentally amplified by PCR and sequenced. The results showed that the fragment was 8 558 bp，and containing all the members of the prfAregulated virulence gene cluster such as prfA, hly, plcA, plcB, mpl and actA. Phylogenetic analysis of the isolate XFL0605 and other reference L. monocytogenes, L. seeligeri and L. ivanovii strains based on the gene sequence demonstrated clearly that the evolutionary relationship of strains were different. It revealed that recombination of LIPI-1 has different assembly process. Furthermore, we predicted the signal peptides and transmember domain and their cleavage positions, and investigated the PrfA DNA binding sites of the LIPI-1 virulence genes. The hlyA gene deduced amino acid of XFL0605 contains a PEST-like sequence at the N-terminus, and a conserved undecapeptide, ECTGLAWEWWR, at the C terminus. It also showed that actA gene of XFL0605 encode 604 amino acids but 105 bp nucleotide missing in proline-rich region, only contains two E/DFPPPPXD/E repeat sequences.