小梅山猪发情周期生殖器官雌激素受体α mRNA定位与相对定量研究

畜牧兽医学报 ›› 2007, Vol. 38 ›› Issue (8): 776-780.doi:

小梅山猪发情周期生殖器官雌激素受体α mRNA定位与相对定量研究

丁利军;刘燕杰;丁家桐

扬州大学动物科学与技术学院，扬州 225009

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-08-25 发布日期:2007-08-25

ERα mRNA Expression in Reproductive Tissues of Meishan Sows during Estrus Period

DING Li-jun;LIU Yan-jie;DING Jia-tong

College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China

Received:1900-01-01 Revised:1900-01-01 Online:2007-08-25 Published:2007-08-25

摘要/Abstract

摘要： 采用地高辛标记探针的原位杂交法（In situ hybridization histochemistry，ISHH）测定小梅山猪发情周期第2、7、12、17天卵巢、子宫内雌激素受体α（Estrogen receptor α, ERα）mRNA的细胞定位与相对表达丰度。结果表明，卵巢中ERα mRNA杂交信号出现在各级卵泡的颗粒细胞，黄体早期部分细胞也有ERα基因微弱表达，卵母细胞未见有阳性染色存在。整个发情周期中卵巢ERα mRNA均呈低水平表达，但在发情周期第17天有上升的趋势。子宫中阳性颗粒主要出现在子宫内膜上皮腺体细胞中，基质部分很少。子宫内膜ERα mRNA丰度在发情周期第17天极显著高于发情周期第12天（P<0.01）。放射免疫测定发情周期第2、7、12、17 天血浆E2水平，与子宫内膜ERα mRNA呈现同步性变化，两者显著相关（r=0.918）。

Abstract: To investigate localization and quantum of ERα mRNA in ovary and uterus of porcine on D 2, 7, 12, 17 throughout menstrual cycle, nonradioactive in situ hybridization using oligonucleotide probes labeled by DIG was applied In the porcine ovary, positive signals for ERα mRNA were limited exclusively to the granulose cells of all types of follicles and were present in a few cells of early corpora lutea. Level of ERα mRNA in ovary remained low during the whole oestrus cycle but increased during proestrus on D 17. ERα mRNA positive signals were mainly detected in glandular epithelial cells and a few of stroma cells in the uterus Abundance of ERα mRNA in porcine endometrium on D 17 was significantly higher than that of D 12 (P<0.01). E₂ concentrations of serum on D 2, 7, 12, 17 during the menstrual cycle were measured by radioimmunoassay. The levels of ERα mRNA in porcine endometrium synchronized the changes of estrogen with positively relations (r=0.918).

丁利军;刘燕杰;丁家桐. 小梅山猪发情周期生殖器官雌激素受体α mRNA定位与相对定量研究[J]. 畜牧兽医学报, 2007, 38(8): 776-780.

DING Li-jun;LIU Yan-jie;DING Jia-tong. ERα mRNA Expression in Reproductive Tissues of Meishan Sows during Estrus Period [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2007, 38(8): 776-780.

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