中国家驴mtDNA D-loop遗传多样性与起源研究

畜牧兽医学报 ›› 2007, Vol. 38 ›› Issue (7): 641-645.doi:

中国家驴mtDNA D-loop遗传多样性与起源研究

葛庆兰;雷初朝;蒋永青;陈宏;张伟;党瑞华;郑惠玲;张爱玲;李天鹏

1．西北农林科技大学动物科技学院陕西省农业分子生物学重点实验室，杨凌 712100；2．徐州师范大学细胞与分子生物学研究所，徐州 221116；3．河南省安阳县畜牧局，安阳 455000

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-07-25 发布日期:2007-07-25

Mitochondrial DNA D-loop Genetic Diversity and Origin of Chinese Domestic Donkeys

GE Qing-lan;LEI Chu-zhao; JIANG Yong-qing;CHEN Hong ;ZHANG Wei;DANG Rui-hua;ZHENG Hui-ling;ZHANG Ai-ling;LI Tian-peng

1.Shaanxi Key Laboratory of Molecular Biology for Agriculture，College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China； 2. Institute of Cellular and Molecular Biology, Xuzhou Normal University, Xuzhou 221116, China； 3. Anyang Animal Husbandry Bureau of Henan Province, Anyang 455000, China

Received:1900-01-01 Revised:1900-01-01 Online:2007-07-25 Published:2007-07-25

摘要/Abstract

摘要： 对我国12个家驴品种126个个体（包括引用26个个体）的mtDNA D-loop 区399 bp进行分析，共检测到36种单倍型37个多态位点，其单倍型多样度为0.466 7~0.977 8，核苷酸多样度为0.001 2~0.028 5，表明我国家驴的遗传多态性丰富。与3条努比亚野驴、3条索马里野驴和6条亚洲野驴的序列构建 NJ系统发育树，首次证明我国家驴的母系起源为非洲野驴中的索马里驴和努比亚驴，亚洲野驴不是中国家驴的祖先。本文还讨论了我国家驴可能的迁徙路线。

Abstract:

All 126 mtDNA D-loop sequences 399 bp in length from 12 Chinese domestic donkey breeds were analyzed, including 26 sequences published before. The results revealed 36 different haplotypes with 37 variable sites.The haplotype diversity and the nucleotide diversity were 0.466 7-0.977 8 and 0.001 2-0.028 5, respectively, indicating high level of genetic diversity in Chinese domestic donkeys. A Neighbor-joining tree constructed using 36 haplotypes of Chinese domestic donkeys, 3 sequences from Nubian wild ass, 3 sequences from Somali wild ass and 6 sequences belonging to Asian wild ass firstly showed that the maternal origin of Chinese domestic donkeys was from Somali and Nubian wild ass in Africa but not from Asian wild ass We also discussed transport of donkeys among different regions in China

葛庆兰;雷初朝;蒋永青;陈宏;张伟;党瑞华;郑惠玲;张爱玲;李天鹏. 中国家驴mtDNA D-loop遗传多样性与起源研究[J]. 畜牧兽医学报, 2007, 38(7): 641-645.

GE Qing-lan;LEI Chu-zhao;JIANG Yong-qing;CHEN Hong;ZHANG Wei;DANG Rui-hua;ZHENG Hui-ling;ZHANG Ai-ling;LI Tian-peng. Mitochondrial DNA D-loop Genetic Diversity and Origin of Chinese Domestic Donkeys[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2007, 38(7): 641-645.

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