畜牧兽医学报 ›› 2007, Vol. 38 ›› Issue (5): 425-431.doi:

• 遗传繁育 • 上一篇    下一篇

猪miR-206前体的克隆、表达及生物信息学分析

陈俭海;魏文娟;肖 骁;谢胜松;冯 阳;赵书红   

  1. 华中农业大学农业动物遗传育种与繁殖教育部重点实验室及农业部猪遗传育种重点开放实验室
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-05-25 发布日期:2007-05-25

Cloning, expression and bioinformatics analysis of miR-206 precursor in the pig

CHEN Jian-hai; WEI Wen-juan;XIAO Xiao; XIE Sheng-song;FENG Yang;ZHAO Shu-hong   

  1. Key Lab of Agricultural Animal Genetics,Breeding and Reproduction of Ministry of Education & Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture, Huazhong Agricultural University, Huazhong Agriculture University
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-05-25 Published:2007-05-25

摘要: 研究骨骼肌中表达的miRNAs对深入探明骨骼肌生长发育的调控机制具有十分重要的意义。本文采用比较基因组学与生物信息学相结合的方法,从猪骨骼肌中克隆了猪miR-206的前体(Precursor),对该miRNA前体的二级结构及与其它物种序列的同源性进行了分析。RT-PCR结果显示,该前体miRNA在猪大多数组织中均有表达,在骨骼肌中表达量最高。生物信息学分析表明miR-206前体序列在不同物种间具有较高的保守性

Abstract: MiRNAs play important roles in skeletal muscle development. In this study, the porcine miR-206 precursor is cloned and sequenced from skeletal muscle through comparative genomics approach. The secondary structure was predicted. RT-PCR results indicated that the miR-206 precursor is expressed in most of the tissues and exhibited the highest expression level in skeletal muscle. Homology analysis revealed that pre-miR-206 is highly conserved among different species.