Abstract: Porcine Endogenous Retrovirus（PERV）is a kind of virus correlatively to the safety of pathogens during pig xenografts. The env gene encodes the Env protein, which plays an important role in virus subtyping, host ranging, cells tropism and mechanism of virus infect, as well as induction of neutralizing antibody secretion. In this research, the envelope gene of PERV was amplified by RT-PCR and sequenced from the peripheral blood lymphocyte of WZS minipigs, The Secondary Structure and B cell Epitopes of the Env Protein of PERV were analysed and predicted subsequently by the Bioinformatics software. The results showed that the PERV-Env protein was supposed to exist18 potential antigen epitopes and 7 potential glycosylated sites. These results indicated that it would be helpful not only to the PERV vaccine designing and the preparation of both of monoclonal antibody and diagnostic reagent, but also to research of Env Protein function and mechanism of PERV infecting human-derived cells.