RNAi表达载体对avUCP基因表达的抑制作用

畜牧兽医学报 ›› 2007, Vol. 38 ›› Issue (4): 352-355.doi:

RNAi表达载体对avUCP基因表达的抑制作用

仲明;张宏福;董枫岚;王润平;刘燕;刘维全;杨琳

1.动物营养学国家重点实验室，北京 100094；2.华南农业大学动物科技学院，广州 510642；3.中国农业大学生物学院，北京 100094

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-04-25 发布日期:2007-04-25

The Inhibitory Effects of siRNA Expression Vector on the Expression of avUCP Gene

Received:1900-01-01 Revised:1900-01-01 Online:2007-04-25 Published:2007-04-25

摘要/Abstract

摘要： 研究RNA干扰（RNA interference, RNAi）表达载体对UCP基因的抑制作用。针对鸡解耦联蛋白（avian uncoupling protein，avUCP）基因，构建4种RNAi质粒表达载体（分别称为A、B、C和D号载体）。再经过脂质体法转染成肌纤维，应用荧光定量PCR及流式细胞术检测其对avUCP mRNA及蛋白表达的影响。构建的4种表达载体均抑制了avUCP的mRNA及蛋白的表达，其中转染C号载体的成肌纤维细胞avUCP mRNA表达相当于空白对照组的14.6%，蛋白抑制率达93.7%。RNAi表达载体可以有效的抑制avUCP基因的表达。

Abstract: To investigate the inhibitory effects of RNAi（RNA interference） expression vector on the expression of avUCP gene. Four siRNA expression vectors (A, B, C and D) were constructed to be aimed directly at avUCP gene. The recombinants were transfected into myofibroblast with liposomes. Expression of avUCP was detected with fluorescence quantitative PCR（FQ-PCR）and flow cytometry. Four kinds of expression vectors could reduce the expression of avUCP mRNA and protein all in myofibroblast. The expression of avUCP mRNA reduced to 14.6% of control group, and the protein inhibition ratio reached 93.7%. The RNAi expression vector can effectively inhibit the expression of avUCP gene.

仲明;张宏福;董枫岚;王润平;刘燕;刘维全;杨琳 . RNAi表达载体对avUCP基因表达的抑制作用[J]. 畜牧兽医学报, 2007, 38(4): 352-355.

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