Abstract: BALB/c mice were inoculated with the recombinant plasmids(pCI-H1-HA，pCAGGS-H1-HA，pCI-H3-HA and pCAGGS-H3-HA) which included the HA gene of H1N1 and H3N2 subtype SIV to screen valuable vaccine candidate. The immune efficacy was evaluated by detecting HI antibody titers，analyzing weight changes of mice and examining virus titers in lungs. The results indicated that the constructed DNA vaccines induced immune responses in BALB/c mice Meanwhile, by the statistical analysis of the weight changes of BALB/c mice, there were high significant differences between the immunization groups and the control(P<0.01), and the DNA vaccines constructed with pCAGGS as the expression vector had better immune efficacy than that of DNA vaccines constructed with pCI(P<0.05).