分泌型和非分泌型表达猪细小病毒VP2蛋白的重组乳酸菌经口免疫小鼠的效果分析

畜牧兽医学报 ›› 2007, Vol. 38 ›› Issue (12): 1405-1409.doi:

分泌型和非分泌型表达猪细小病毒VP2蛋白的重组乳酸菌经口免疫小鼠的效果分析

徐义刚;唐丽杰;崔丽春;赵丽丽;李一经

1东北农业大学动物医学学院，哈尔滨 150030；2东北林业大学，哈尔滨150040

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-12-25 发布日期:2007-12-25

Analysis of Immune Responses Induced by Recombinant Secretion and Non-secretion Type Lactobacillus casei Expressing Porcine Parvovirus VP2 Protein via Oral Immunizations

1. College of Veterinary Medicine,Northeast Agricultural University,
Harbin 150030，China; 2. Northeast Forestry University, Harbin 150040，China

1. College of Veterinary Medicine,Northeast Agricultural University, Harbin 150030，China; 2. Northeast Forestry University, Harbin 150040，China

Received:1900-01-01 Revised:1900-01-01 Online:2007-12-25 Published:2007-12-25

摘要/Abstract

摘要： 对比分析在不同细胞部位表达猪细小病毒（PPV）主要免疫保护性抗原VP2蛋白的重组干酪乳杆菌系统作为口服疫苗的免疫效果。将构建的细胞表面表达和分泌表达猪细小病毒VP2蛋白的重组干酪乳杆菌分别经口免疫BALB/c小鼠，免疫分3次进行，时间间隔为2周，每次连续接种3 d，每只小鼠每次接种100 μL 1010CFU/mL的菌量，对照组小鼠接种相同剂量的PBS。初免后不同时间收集免疫小鼠粪便及肠黏液样本测定小鼠产生抗PPV的特异性sIgA抗体水平，采集小鼠血液样本测定其血清中抗PPV的特异性IgG抗体水平。间接ELISA检测结果表明，两种表达系统均能诱导小鼠产生黏膜及系统免疫应答，分泌型的重组菌系统免疫小鼠诱导机体产生的抗PPV的特异性sIgA和IgG抗体水平高于细胞表面表达型的重组菌系统的免疫效果，表明分泌型的重组乳酸菌作为活菌疫苗具有更好的免疫性。

Abstract: The objective of this research was to compare the immune responses induced by mucosal immunizations with recombinant secretion and nonsecretion type Lactobacillus casei expressing porcine parvovirus (PPV) protective antigen VP2 protein. The mice of vaccine groups received recombinant L. casei strains constructed in this work harboring cell surface expression system pPG-VP2 or secretion expression system pPGS-VP2, respectively. The control mice were immunized with equivalent doses of PBS. All the mice were immunized via orogastricroute. The immune protocol was administered on three consecutive days at day 0, 1 and 2. A booster immunization was given at day 14, 15 and 16 and a second booster was given at day 28, 29 and 30. Specific anti-PPV-VP2 secret immunoglobulin A (sIgA) antibody in the feces and intestines mucus and anti-PPV-VP2 IgG antibody in the serum of immunized mice was detected by indirect enzyme linked immunosorbent assay (ELISA) after orogastric immunization. The results showed that the levels of mucosal and systemic immune responses induced by secretion type recombinant lactobacilli were higher than that induced by cell surface expression type recombinant lactobacilli.

徐义刚;唐丽杰;崔丽春;赵丽丽;李一经. 分泌型和非分泌型表达猪细小病毒VP2蛋白的重组乳酸菌经口免疫小鼠的效果分析[J]. 畜牧兽医学报, 2007, 38(12): 1405-1409.

. College of Veterinary Medicine;Northeast Agricultural University;Harbin 000;China; . Northeast Forestry University;Harbin 000;China. Analysis of Immune Responses Induced by Recombinant Secretion and Non-secretion Type Lactobacillus casei Expressing Porcine Parvovirus VP2 Protein via Oral Immunizations[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2007, 38(12): 1405-1409.

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