Abstract: Primary culture of porcine preadipocytes was established using fat tissue from an adult pig. The preadipocytes to re-differentiate induction into mature adipocytes was confirmed by oil red O staining. A porcine preadipocyte (PreA) lines were transfected with pEGFP-N1 plasmid DNA by lipofectinmediated way. GFPPreA clones were obtained by G418 selection. PreA, fetal fibroblast (FF), and GFP-PreA were used as nuclear donors for somatic cell nuclear transfer in pig. The results were as follows: For the blastocyst rate of cloned embryos, no significant difference was observed between PreA and FF (8.8% vs 8.3%, P > 0.05). Blastocyst rate of reconstructed with GFP transfected PreA decreased in comparison to those derived from nontransgenic PreA.（3.7% vs. 8.8%，P > 0.05）。The results indicate that GFP transfected PreA can produce procine transgenic blastocytes. In spite of low blastocyst rate, cloning pigs from preadipocyte is possible.