Abstract: The difference of gene expression in chicken sperm storage tubules (SST) was investigated by differential display reverse transcriptase polymerase chain reaction (DDRT-PCR). One specific fragment, named SST01, was found. After secquencing and BLAST analysis, SST01 displayed high homology (98%) to neurexophilin gene of chicken. Neurexophilin is a glycoprotein which is detected only from neurons, which indicated that nervous system be related to the storage or release of sperm in SST. Neurexophilin functions as the ligand of neuridine which can cause cytoadherence, which indicated that neurexophilin be related to the storage of sperm in SST.