Abstract: The effect supplementing with Chinese Medicine effective constituents Baicalin（Bai）and Ligustrazine（Lig） into culture medium on 2-cell mouse embryos in vitro culture was compared in this study. And the morula and blastocyst in vitro culture were conserved through two freezing process The frozenthawed modality choiceness embryos were cultured to blastocyst by 8-14 h or 6-8 h and transferred to recipient uterus. The results showed that the hatching rate of embryos at 120 h from Bai(80.6%) and Lig(78.3% ) were significant higher than control group (51.8%), respectively (P<0.01)According to the cell amount of embryos hatched, the difference between experiment groups Lig(83.9±7.7) ,Bai(81.9±6.2) and control group（77.4±5.6） were extremely significant (P<0.01).The development rates of morula cultured for 8-14 hours after frozenthawed in two freezing process, the Bai and the Lig groups were significantly higher than the control group. The livability of Blastocyst cultured for 6-8 hours after frozenthawed in two freezing process, the Bai and the Lig groups were better than the control group.The recipient pregnancy rates, littering rates of embryos, initial weight of baby mouse, mean weight of away cote and living rate of away cote of Bai and Lig groups were better than control group. These results indicated the two Chinese Medicine effective constituents can improve 2-cell mouse embryo quality in vitro culture and make for the developmental potential of frozenthawed transfer embryos.