中国美利奴羊MHC-DRB1基因PCR-RFLP多态性分析

畜牧兽医学报 ›› 2007, Vol. 38 ›› Issue (10): 1115-1119.doi:

中国美利奴羊MHC-DRB1基因PCR-RFLP多态性分析

彭林泽;申红;贾斌;余智勇;曾现存;刘宏坤;彭波;陈明辉;郭玉强

1石河子大学动物科技学院，石河子 832003；2新疆伊犁农四师74团畜牧兽医站，伊犁 835600；3新疆塔城农九师畜牧兽医站，塔城 834600

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-10-25 发布日期:2007-10-25

Polymorphism Analysis of MHC-DRB₁ Gene in Chinese Merino Sheep by PCRRFLP

PENG Lin-ze;SHEN Hong;JIA Bin;YU Zhi-yong;ZENG Xian-cun;
LIU Hong-kun;PENG Bo;CHEN Ming-hui;GUO Yu-qiang

1College of Animal Science & Technology ,Shihezi University,Shihezi 832003, China;2Animal Husbandry and Veterinary Station at Regimental Farm 74 in Xinjiang,Yili 835600, China;3Animal Husbandry and Veterinary Station at Agricultural Construction Division 9 in Xinjiang,Tacheng 834600, China

Received:1900-01-01 Revised:1900-01-01 Online:2007-10-25 Published:2007-10-25

摘要/Abstract

摘要： 应用巢式PCRRFLP方法，对211只中国美利奴（新疆军垦型）羊的MHCDRB1外显子2的遗传多态性进行检测,并对基因型频率和等位基因频率进行了统计，结果表明，中国美利奴（新疆军垦型）羊的MHC-DRB1基因外显子2在SacⅠ、Hin1Ⅰ和HaeⅢ的酶切位点存在多态性，这些酶切位点分别受2、2和6个等位基因控制，由于MHC-DRB1为多碱基突变的基因位点，综合3种限制性内切酶的酶切结果，在中国美利奴（新疆军垦型）羊中共发现24种等位基因；χ2适合性检验结果表明，中国美利奴（新疆军垦型）羊的MHCDRB1外显子2在SacⅠ和Hin1Ⅰ酶切位点均达到Hardy-Weinberg平衡（P＞0.05），但在HaeⅢ酶切位点未达到HardyWeinberg平衡（P＜0.01）。

Abstract: The polymorphism of the exon 2 of MHC-DRB₁ was investigated by nested PCR-RFLP technique at three loci digested with SacⅠ,Hin1Ⅰand HaeⅢ,respectively, in a total number of 211 individuals of Chinese Merino(Xinjiang Army Wasteland Reclamation) sheep, and genotypic frequencies and allelic frequencies were counted. The results showed that the extensive polymorphisms of the exon 2 of MHC-DRB1 were found at three loci digested with SacⅠ,Hin1Ⅰ and HaeⅢ,respectively, in Chinese Merino sheep, these sites were controlled by two, two and six alleles, respectively. As some base mutations were found in MHCDRB1 locus, and according to the results in this paper, twentyfour alleles were found in Chinese Merino(Xinjiang Army Wasteland Reclamation) sheep. The results of χ² test showed that genotypes of MHC-DRB1 digested with SacⅠand Hin1Ⅰexcept HaeⅢ（P＜0.01）fitted with Hardy-Weinberg equilibrium(P＞0.05)

彭林泽;申红;贾斌;余智勇;曾现存;刘宏坤;彭波;陈明辉;郭玉强. 中国美利奴羊MHC-DRB1基因PCR-RFLP多态性分析[J]. 畜牧兽医学报, 2007, 38(10): 1115-1119.

PENG Lin-ze;SHEN Hong;JIA Bin;YU Zhi-yong;ZENG Xian-cun;LIU Hong-kun;PENG Bo;CHEN Ming-hui;GUO Yu-qiang. Polymorphism Analysis of MHC-DRB₁ Gene in Chinese Merino Sheep by PCRRFLP[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2007, 38(10): 1115-1119.

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