Abstract: The objective of this study was to investigate the changes of the number of lymphocyte, goblet cell reside within the intestinal musocal epithelium and mast cells’ density within intestinal wall area in beef calf.Histochemical and the Scion image software analysis techniques were used. Data were collected from duodenum, jejunum and ileum of 4 beef Limousin crossbred calves at 1-, 4- and 6-month of age, respectively.Results showed that intraepithelium lymphocyte number and mast cells’ density gradually decreased, but goblet cell number gradually increased from duodenum, jejunum to ileum in beef calf at same month of age.Intraepithelium lymphocyte number of duodenum, jejunum and ileum in beef calf, which significantly differed （P<0.01）each other at 1- and 6-month of age, intraepithelium lymphocyte number of duodenum at 4-month of age was the mostGoblet cell number of duodenum, jejunum and ileum in beef calf was significantly different （P<0.01）each other at 1-month of age.At 4- and 6-month of age, goblet cell number didn’t differ significantly（P>0.05） between duodenum and jejunum, but the numbers of these two segments were less （P<0.01）than that of ileum.As for mast cells’ density within intestinal wall area of duodenum, jejunum and ileum in beef calf,they significantly differed （P<0.01）each other at 1-, 4- and 6-month of age, respectively, mast cells’ density within intestinal wall area was least at 4month of age.Our results indicated immune responses may involve the coordination and potentially redundant development of calf.