畜牧兽医学报 ›› 2007, Vol. 38 ›› Issue (10): 1102-1108.doi:

• 基础兽医 • 上一篇    下一篇

山羊胎儿脊神经节发育过程中NGF及其受体TrKA的表达

卿素珠;林吉茂;徐永平;张涌   

  1. 西北农林科技大学动物科技学院,杨凌 712100
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-10-25 发布日期:2007-10-25

Expression of NGF and TrKA in Developing Dorsal Root Ganglia of Goat Fetus

QING Su-zhu;LIN Ji-mao;XU Yong-ping;ZHANG Yong   

  1. College of Animal Science and Technology, Northwest A & F University, Yangling 712100,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-10-25 Published:2007-10-25

摘要: 山羊胎儿脊神经节于胚胎第6周就已形成,但此时节内神经元形态不清晰,至第8周,节内神经元形态逐渐清晰,节内神经纤维数量少;随着胚胎发育,节内神经元数量并不显著增加,而是节细胞之间神经纤维及胶质细胞的增多和神经元个体的增长,从而使神经节的体积逐渐增大。节内神经元既可分为形态和着色不同的明暗2类,也可分为细胞直径不同的大、中、小3类。山羊胎儿脊神经节内存在NGF及其受体TrKA,随着胚胎发育,NGF主要分布于节内各类神经元的胞质中,特别是在第14周后;TrKA阳性反应则在第10周后才出现,主要分布于中、小神经元的胞质和胞核,而在大神经元未能见到阳性反应,但在大神经元周围的卫星细胞可观察到TrKA强阳性反应产物。结果提示,NGF对外周神经嵴起源的发育中感觉神经元的生理功能具有重要的调节作用,并与卫星细胞的生长发育和成熟有关。

Abstract: The dorsal root ganglia (DRG) of goat fetus formed before 6th embryonic week, but the morphology of neurons inside ganglia were not very distinct until 8th embryonic week. The increase of ganglion volume mainly lied in growth of neurons and increasing of nerve fibers and neuroglia cells, but not lied in marked increase of neurons in number. Neurons could be divided into dark subpopulation and bright subpopulation according to their shape and staining extent, or three subpopulation—large size, medial size and small size according to cellular diameter. NGF and TrkA immunoreactivity were present in DRG of goat fetus. NGF-IR mainly localized in cytoplasm of three subpopulation, especially after 14th embryonic week; TrkA-IR occurred after 10th embryonic week, and were mainly distributed in cytoplasm and nucleus of medial-sized and smallsized neurons. No TrkA-IR could be detected on large-sized neurons, but TrkA immunostaining distributed in satellite cells surronding large-sized neurons The results suggested that NGF play an important role both in regulating the physiological function of developing sensory neurons derived from peripheral neural crest and in growth,development and maturation of satellite cells.