Abstract:

Polymorphisms in exon 14 of Mx1 gene were detected by PCRRFLP in Sutai pigs.Six genotypes and three alleles were observed when exon 14 of Mx1 gene was digested by Hin6Ⅰrestriction enzyme. The results of Chi Square test indicated that the frequencies patterns of exon 14 of Mx1 gene were fit with Hardy-Weinberg equilibrium in Sutai pigs population. Correlation between genotypes and reproductive performances (total litter size, number born alive, birth litter weight, number weaning piglets and weaning litter weight in the third litter) of Sutai pigs was similarly analyzed. The results indicated that reproductive performances of Sutai pigs with BC genotype were significantly (P<0.05）higher than those with BB, AA, AB, AC and CC genotypes. Three new points mutation were identified by sequencing PCR products, two of which resulted in Thr→Ala and Glu→Arg were missense mutation, and the last was a silence mutation. Glu→Arg point mutation and silence mutation were appeared in the individuals with BB genotype. The results also indicated that the point mutation Glu→Arg may influence the virus resistance of Mx protein in pigs