Abstract: A recombinant pseudorabies virus (PRV) (rPRV-HA) expressing the hemagglutinin (HA) gene from H3N2 subtype swine influenza virus (SIV) was evaluated in a mouse model. A total of 150 8-week-old female BALB/c mice were used in this study. The mice were each inoculated intranasally with 10^5.0 TCID₅₀ of rPRV-HA (rPRV-HA group, n=60) or attenuated vaccine Bartha-K61 (Bartha-K61 group, n=60), and another two unimmunized groups served as challenged group (n=20) or unchallenged group (n=10). Some mice in rPRV-HA and Bartha-K61 groups were sacrificed for antigen detection and virus isolation at different days post-immunization (DPI), and the others were each challenged with 10^5.0 TCID₅₀ of the same subtype SIV 28 DPI. Recombinant virus could be detected in the lung of rPRV-HA-immunized mice. Antibody responses to PRV were detected by indirect immunofluorescence assay, but not by seroneutralization test, in both rPRV-HA and Bartha-K61 groups. SIV-specific antibodies were detected by hemagglutination inhibition test only in rPRV-HA group 14 DPI. The rPRV-HA-immunized mice were protected from homologous SIV challenge, as indicated by limiting virus replication and pathological changes of the organs, and boosted antibody responses to SIV post-challenge.