畜牧兽医学报 ›› 2006, Vol. 37 ›› Issue (12): 1368-1372.doi:

• 研究简报 • 上一篇    下一篇

不同电融合条件对莎能奶山羊转基因(GFP)核移植胚胎发育的影响

王亮;彭涛;刘婷婷;汪汉卿;曹旭;朱海;吕自力;张达江;蔡涛;高红   

  1. 1.中国科学院兰州化学物理研究所甘肃省天然药物重点实验室,兰州 730000;2.新疆金牛生物科学院,乌鲁木齐 830026;3.中国科学院新疆分院理化技术研究所,乌鲁木齐 830011;4.中国科学院研究生院,北京,100039
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2006-12-25 发布日期:2006-12-25

Influence of Different Electricfusion Conditions on the Development of ransgenic Nuclear Transfer Embryos of Saanen Dairy Goat

  • Received:1900-01-01 Revised:1900-01-01 Online:2006-12-25 Published:2006-12-25

摘要: 以莎能奶山羊(Saanen dairy goat) 转基因(GFP)成纤维细胞为核供体细胞,以普通山羊卵母细胞为受体细胞进行了核移植,试验中对转基因核移植胚胎的电融合条件进行了优化,发现对于莎能奶山羊转基因(GFP)重构胚胎的处理,电融合时方案B(1.5kV/cm、 30μs/次、间隔1s)电融合参数最适合用于莎能奶羊的转基因(GFP)核移植胚胎,其重构卵电融合率为89.4%,重构胚分裂率为68.0%。

Abstract: In this study using the transgenic (GFP) fibroblasts of Saanen dairy goat as donor cells and using the abattoirderived oocytes of normal goat as recipient cells the nuclear transfer experiments had been processed. During experiments the optimization of electricfusion experiment parameters of transgenic nuclear transfer embryos had been done. For the treatment of transgenic reconstruction embryos of Saanen dairy goat, the electricfusion parameter of group B in experiment plans (1.5kV/cm for 30 μs and pulse interval for 1s) is most suitable for the transgenic reconstruction embryos of Saanen dairy goat, the electricfusion rate of 89.4% for cell-cytoplast complexes and the cleavage rate of 68.0% for reconstructed embryos have been obtained.