Abstract: The full-length genome of PRRSV HB-2(sh)/2002 was sequenced and analyzed. The size of the virus genome was 15 373 nucleotides, excluding the poly(A)tail. Comparative analysis with the genomic sequences of domestic and abroad North American isolates showed that HB2(sh)/2002 shared 88.7%~95.1% identity with them. The isolate was a mutant of PRRSV with unique deletion, namely a 36 nucleotides deletion in Nsp2 and 3 nucleotides deletion in GP3 were found. This is first finding that PRRSV exists deletion variation. Our results enrich information data of PRRSV genome and provide a valuable basis for further study of biological characteristics, heredity and mutation of PRRSV.