Abstract: Upstream regulatory region of 14 alleles were studied for polymorphism in BoLA-DQB loci. URRs of each allele were amplified by the polymerase chain reaction. The PCR products were cloned and sequenced. Alignment of the URR sequences showed that each DQB·exon2 alleles linked with 2 different URR sequences, thus, of 14 DQB alleles analyzed, a total of 15 different DQB-URR were found. Regulatory elements W box，X box，Y box，CCAAT box and TATAlike box which were associated with the expression of structural gene were found in all the URR sequences. W box，X box，Y box and TATA-like box were polymorphic, CCAAT box was conserved. Polymorphic sites also existed between the regulatory elements. This perhaps affected the level of expression of structural genes.