畜牧兽医学报 ›› 2005, Vol. 36 ›› Issue (4): 387-390.doi:

• 兽医 • 上一篇    下一篇

捻转血矛线虫(H.contortus)ZJ株H11蛋白基因的克隆及序列分析

杜爱芳;李孝军;侯玉慧;王素华   

  1. 浙江大学动物预防医学研究所,杭州 310029
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2005-04-25 发布日期:2005-04-25

Cloning and Sequence Analysis of the H11 Antigen Gene of Haemonchus contortus ZJ strain

DU Ai-fang;LI Xiao-jun;HOU Yu-hui;WANG Su-hua   

  1. Institute of Preventive Veterinary Medicine,Zhejiang University,Hangzhou 310029,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2005-04-25 Published:2005-04-25

摘要: 参照已发表的捻转血矛线虫H11蛋白基因序列设计引物,以H.contortus ZJ株的总RNA为模板,进行RT-PCR扩增,成功扩增出H11基因。将PCR产物与pUCm-T载体连接后,转化DH5α感受态细胞,筛选阳性克隆并测序。序列分析表明,其核苷酸序列与国外报道的H11基因的同源性为99.5%。编码氨基酸序列具有氨基肽酶的保守序列,推测可使虫体不能获得所必需的氨基酸而导致死亡。

Abstract: In order to clone the H11 gene, the total RNA of Haemonchus contortus ZJ strain was extracted and used as template of RT-PCR. A pair of primers was designed according to the published data. After RT-PCR, the cloned H11 was ligated to the vector pUCm-T and transformated DH5α competent cell. The positive clones were verified by sequencing. Sequence analysis showed that the gene obtained was 99.5% identical to the H11 gene in the database. According to the sequence analysis, the deduced protein of H11 may has the function similar to aminopeptidase which make parasites dead from lack of necessary amino acids.