畜牧兽医学报 ›› 2005, Vol. 36 ›› Issue (2): 198-201.doi:

• 研究简报 • 上一篇    下一篇

伊氏锥虫多种克隆群体的采集及血清学检测

栗利芳; 袁志波;索 勋; 汪 明;孔繁瑶
  

  1. 中国农业大学动物医学院, 北京 100094
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2005-02-25 发布日期:2005-02-25

Collection of Clonederived Populations of Trypanosoma evansi Stock YN andDetermination of its Variable Antigenic Types by IFT and ABCELA

LI Li-fang;YUAN Zhi-bo;SUO Xun;WANG Ming;KONG Fan-yao   

  1. Parasitology Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing 100094, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2005-02-25 Published:2005-02-25

Abstract: In order to find out the regularity of antigenic variation, 107 cloned population of Trypanosoma evansi stock YN was inoculated sequentially into 5 New Zealand rabbits during 165 days. Blood was collected from the rabbits at 3-day intervals after infection. Ten clone-derived populations were isolated from each; twenty VATs were determined from the 50 populations with the indirect fluorescent antibody staining technique (IFT) and ABC-enzyme-linked assay (ABC-ELA) and designated as BeTat 1.1, BeTat 1.2,…BeTat 1.20.