二肽在断奶仔猪小肠刷状缘膜囊中水解状况的研究

畜牧兽医学报 ›› 2005, Vol. 36 ›› Issue (2): 133-136.doi:

二肽在断奶仔猪小肠刷状缘膜囊中水解状况的研究

代建国;李德发;朴香淑;潘宝海;龚利敏

中国农业大学动物科技学院，北京 100094

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2005-02-25 发布日期:2005-02-25

Hydrolysis of Dipeptides in Small Intestinal Brush Border Membrane Vesicles of Weaned Piglets

DAI Jian-guo; LI De-fa; PIAO Xiang-shu; PAN Bao-hai; GONG Li-min

College of Animal Science and Technology, China Agricultural University, Beijing 100094,China

Received:1900-01-01 Revised:1900-01-01 Online:2005-02-25 Published:2005-02-25

摘要/Abstract

摘要： 通过孵育试验研究了甘-L-亮（Gly-Leu）和L-组-L-亮（His-Leu）2种二肽在断奶仔猪小肠刷状缘膜囊（Brush Border Memlorane Vesides，BBMV）中的水解状况。结果表明，孵育液pH值（分别为3.5、4.0、4.5、5.0、55、6.0、6.5、7.0、7.5和8.0）、孵育温度（25 ℃和37 ℃）、孵育时间（05、1、2、5、10、20、30和40 min）以及BBMV（二肽酶）的添加量均不影响此2种二肽在断奶仔猪小肠BBMV孵育体系中的水解。结果提示，Gly-Leu和His-Leu 2种二肽在断奶仔猪小肠BBMV体系中均不水解。

Abstract: In vitro experiments were carried out to investigate the effects of incubation solution pH, incubation temperature, incubation time and the addition amount of BBMV (presumably dipeptidase) on hydrolysis of dipeptides. The results indicated that incubation solution pH (3.5，4.0，4.5，5.0，5.5，6.0，6.5，7.0，7.5 and 8.0) , incubation temperature (25 ℃ and 37 ℃) , incubation time (0.5, 1, 2, 5, 10, 20, 30 and 40 min) and the addition amount of BBMV were not effective on hydrolysis of the dipeptides. The results suggested that these two dipeptides (Gly-Leu and His-Leu) were not hydrolyzed in small intestinal BBMV of weaned piglets.

代建国;李德发;朴香淑;潘宝海;龚利敏. 二肽在断奶仔猪小肠刷状缘膜囊中水解状况的研究[J]. 畜牧兽医学报, 2005, 36(2): 133-136.

DAI Jian-guo; LI De-fa;PIAO Xiang-shu; PAN Bao-hai; GONG Li-min. Hydrolysis of Dipeptides in Small Intestinal Brush Border Membrane Vesicles of Weaned Piglets[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2005, 36(2): 133-136.

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