Abstract: Nanyang and Luxi cattle were used as experimental population; the genetic variations of GH genes were researched by PCR-SSCP and PCR-RFLP method. The results were as follows: The polymorphic PCR-SSCP sites in the 3^rd, 4^th intron, the 5^th exon, and 5′flanking region and the polymorphic HaeIII site in the 3′flanking region of the bovine growth hormone (GH) were examined in two breeds. The polymorphisms of the 3rd intron were caused by C→T transition at 1 547 bp position. The polymorphisms of the 4^th intron were tested by T→G transition at 1 947 bp position in Nanyang, Luxi cattle. The polymorphisms of the 5^th exon were caused by C→G transition at 2 141 bp position, which resulted amino acid translation, leucine to valine. And the polymorphic HaeIII site in the 3′flanking region were caused by GCC→GGC transition at 2 639 bp position, and C→T transition at 303 bp resulted the polymorphisms of 5′flanking region.