Abstract: This report describes construction of specific labeling vector in CES cell. The putativeU3-R sequence regions of 1.2 kb of chicken Embryonic Normal Stem cell gene 2 was amplified by PCR and successfully sequenced. Based on sequence analysis and alignments of nucleotides sequence, found many factor or protein binding sites such as AP-1 ,GATA-1and an important elements: B region which exhibits a strong enhancer activity in CES and differentiated cells. The CMV promoter was cut off from pEGFP-N1, the putative U3-R fragment was inserted just upstream of GFP . Results of restriction digestion and DNA sequencing showed that the vector for expressing in CES cell was successfully constructed.