Abstract: Metabolism and deposition of exogenous DNA from transgenic glyphosate-tolerant soybean meal in grower pigs were studied in the experiment. The conventional and transgenic soybean meal diets were fed to barrows fitted with a simple T-cannula at distal ileum for collection of ileal chyme and feces. In addition, feeding experiment with 30% of conventional or transgenic soybean meal was performed in grower for 42 d. Then the tissue samples were obtained after slaughter for detection of exogenous DNA. The results showed that the exogenous gene cp4 epsps derived from glyphosate-tolerant soybean meal was detected in chyme and feces both only at 6.7%, which indicated that integrity of exogenous DNA was greatly or utterly devastated in distal intestine. Based on 5 pg of limit by PCR, there were no deposition of cp4 epsps gene in liver, muscle, spleen and thymus tissue of grower, and this implied that exogenous DNA has already been metabolized and degraded.