Abstract: The ORF2 of porcine circovirus type 2 and BVP22 of bovine herpesvirus were amplified by PCR. The cloned ORF2 gene was inserted into eukaryotic expression vector pCDNA3.1(+)for constructing nucleotide vaccine vector pCORF2. Then nucleotide vaccine vectors which express BVP22-ORF2 fusion protein were constructed by inserting BVP22 gene,which has the function of protein transduction into pCORF2 at the upstream or downstream of the ORF2 gene respectively. All the above vaccines were intramuscular injected to BALB/c mice for twice at two weeks interval. 2 and 6 weeks after the first immune injection, serums were collected and the humoral antibodies were tested by ELISA. The result of ELISA showed that all the experimental groups produced anti-ORF2 antibodies, and high antibody level showed four weeks after the second immune injection. Results also showed that BVP22 could enhance the efficiency of ORF2 nucleotide vaccine. Our research provided a candidated vaccine for controlling PCV2 infection.