Abstract: By cotransfection of PRV Fa SA215 DNA and PP63LacZE2 DNA in Vero cells,12 recombinants named SA215(A)1,SA215(A)2…，and SA215(A)12 were obtained with calcium phosphate transfection system. The recombinant strains were determined preliminarily by biotin-labeled probe of HCV E2， then SA215(A)1 strain were identified by BamHI digestion and Southern-blot acid hybridization. The results indicated that the recombinant virus of PRV and HCV named SA215(A) was successful. IFT，SDS-PAGE，electrophoresis and Western-blot all confirmed that HCV E2 gene was expressed in recombinant virus, about 51 ku fragment of envelope glycoprotein E2 were acquired. Research on culture characteristics of SA215(A) strain indicated recombinant virus could be replicated very well on Vero cells, BHK21 cells and so on, but showing variability in culture on different cell lines. 21-day-old SPF pigs were inoculated with SA215(A) strain. After 28 days, the pigs were affected with 10⁶PFU high-dose virulent PRV Fa strain; and 14 days later affected with 10⁵ MLD high-dose virulent HCV SM strain. All of the inoculated pigs were protected. The results indicated SA215(A) was high efficacy.