Abstract: Effects of wheat NSP and xylanase on intestinal microflora and bacterial community in broilers were determined by traditional method and denaturing gradient gel electrophoresis (DGGE) analysis of 16S ribosomal RNA. 90 AA broilers were allocated randomly into 3 groups, fed 3 diets separately. The diets were corn based, wheat-based or wheat-based supplemented with xylanase. The results showed that the wheat NSP significantly increased (P<0.05) the microbial population of total anaerobic bacteria in cecum or ileum and increased (P<0.05) the streptococcus faecalis in cecum of broilers. However, the wheat NSP significantly decreased (P<0.05) the bacterial community in intestine of broilers. The xylanase-supplemented diet had no markedly effect (P>0.05) on microbial population and bacterial community in cecum and ileum of broilers.