氨气和硫化氢应激对肉羊免疫及抗氧化功能的影响

doi:10.11843/j.issn.0366-6964.2018.10.015

摘要/Abstract

摘要：

旨在研究不同浓度氨气和硫化氢应激对肉羊免疫及抗氧化功能的影响。本研究选用8只青年母羊，随机分为两组，每组4个重复，两组羊在动物营养代谢环控舱A舱、B舱内循环进行如下处理，A舱：对照（0~6 d）：3 mg·m^-3NH₃+0 mg·m^-3H₂S；处理1（8~13 d）：20 mg·m^-3NH₃+8 mg·m^-3H₂S；处理2（15~20 d）：40 mg·m^-3NH₃+8 mg·m^-3H₂S；处理3（22~27 d）：80 mg·m^-3NH₃+8 mg·m^-3H₂S；B舱：处理4（8~13 d）：20 mg·m^-3NH₃+16 mg·m^-3H₂S；处理5（15~20 d）：40 mg·m^-3NH₃+16 mg·m^-3H₂S；处理6（22~27 d）：80 mg·m^-3NH₃+16 mg·m^-3H₂S。每个处理试验期6 d。于每个处理试验期间第2和第6天采集羊只血清样，测定指标包括：免疫指标（血清免疫球蛋白、补体、溶菌酶和细胞因子含量）和抗氧化指标（血清总抗氧化能力、抗氧化酶活性和丙二醛含量）。结果表明：1）与对照组相比，入舱第2天，处理3、4血清IL-1β含量显著升高（P<0.05），处理4、6血清IL-6含量极显著升高（P<0.01）；入舱第6天，试验处理组血清IgA、IgG（处理1除外）、IgM含量、血清溶菌酶含量及补体C3、C4水平极显著降低（P<0.01），处理4血清IL-1β含量显著升高（P<0.05）。H₂S浓度对入舱第2天羊血清中LZM、IL-10含量影响显著（P<0.05），对IL-6含量影响极显著（P<0.01）；对入舱第6天羊血清中IL-1β含量、C4水平影响显著（P<0.05），对IgG、IgM、IL-10含量、C3水平影响极显著（P<0.01）；随着入舱时间的延长，血清IgG、IgM含量、C3、C4水平呈下降的趋势；NH₃浓度与H₂S浓度对入舱第2天羊血清中IL-1β含量有显著的互作效应（P<0.05），对IgG、IL-6含量有极显著的互作效应（P<0.01）；对入舱第6天羊血清中IL-1β含量有显著的互作效应（P<0.05）。2）与对照组相比，入舱第2天，处理3、4、5、6血清SOD活性极显著降低（P<0.01），处理4、5、6血清CAT活性极显著降低（P<0.01）；入舱第6天，试验处理组（处理1除外）血清SOD、CAT活性极显著降低（P<0.01），处理5血清T-AOC活性显著降低（P<0.05），处理4、5、6血清MDA含量极显著升高（P<0.01）。H₂S浓度对入舱第2天羊血清中GSH-Px活性、MDA含量影响显著（P<0.05），对血清SOD、CAT活性影响极显著（P<0.01），对入舱第6天血清T-AOC、SOD、CAT活性、MDA含量影响极显著（P<0.01）；NH₃浓度对入舱第2天羊血清中SOD、CAT活性影响显著（P<0.05），对入舱第6天羊血清中SOD、CAT活性影响极显著（P<0.01）；随着入舱时间的延长，血清SOD、CAT活性呈下降的趋势；NH₃浓度与H₂S浓度对入舱第2天羊血清中T-AOC活性有显著的互作效应（P<0.05），对入舱第2天羊血清中SOD、CAT、GSH-Px活性有极显著的互作效应（P<0.01）；对入舱第6天羊血清中SOD、CAT活性有极显著的互作效应（P<0.01）。本研究表明，NH₃和H₂S可引起肉羊应激，降低肉羊免疫功能和抗氧化能力，且随着NH₃与H₂S浓度的升高和作用时间的延长，对机体损害程度加重。

Abstract:

This experiment was conducted to study the effects of ammonia and hydrogen sulfide stress on immunity and antioxidant function of goats. Eight young female goats were randomly divided into two groups, and each group had 4 replicates. The goats were in the separated environmental controlled chamber A and B processed by the following treatments successively. Chamber A:control group (0-6 d):3 mg·m^-3NH₃+0 mg·m^-3H₂S; treatment 1 (T1) (8-13 d):20 mg·m^-3NH₃+8 mg·m^-3H₂S; treatment 2 (T2) (15-20 d):40 mg·m^-3NH₃+8 mg·m^-3H₂S; treatment 3 (T3) (22-27 d):80 mg·m^-3NH₃+8 mg·m^-3H₂S. Chamber B:treatment 4 (T4) (8-13 d):20 mg·m^-3NH₃+16 mg·m^-3H₂S; treatment 5 (T5) (15-20 d):40 mg·m^-3NH₃+16 mg·m^-3H₂S; treatment 6 (T6) (22-27 d):80 mg·m^-3NH₃+16 mg·m^-3H₂S. The serum samples of goats were collected at 2nd and 6th day of each experiment which lasted for 6 days, then the immune indexes (immunoglobulin, complement, lysozyme and cytokine content) and antioxidant indexes (T-AOC, antioxidant enzyme activity and MDA content) were measured. The results showed as follows:1) Compared with the control group, serum IL-1β content in T3, T4 significantly increased (P<0.05), and serum IL-6 content in T4, T6 significantly increased (P<0.01) at the 2nd day; contents of serum IgA, IgG, IgM, LZM, C3 and C4 significantly decreased (P<0.01) in the treatments (except T1) and serum IL-1β content significantly increased (P<0.05) in T4 at the 6th day. H₂S concentration had the significant influence on the contents of serum LZM (P<0.05), IL-10 (P<0.05), IL-6 (P<0.01) at the 2nd day, and the contents of serum IL-1β (P<0.05), IgG (P<0.01), IgM (P<0.01), IL-10 (P<0.01), C3 (P<0.01), C4 (P<0.05) at the 6th day. With the increase of time in climate chambers, contents of serum IgG, IgM, C3, C4 decreased. NH₃ and H₂S concentrations had the significant interaction effect on contents of serum IL-1β (P<0.05), IgG (P<0.01), IL-6 (P<0.01) at the 2nd day and IL-1β (P<0.05) at the 6th day. 2) Compared with the control group, the activities of serum SOD in T3, T4, T5, T6 and CAT in T4, T5, T6 significantly decreased (P<0.01) at the 2nd day; at the 6th day, the activities of serum SOD, CAT in the treatments (except T1) significantly decreased (P<0.01), the activity of serum T-AOC in T5 significantly decreased (P<0.05), as well as the serum level of MDA significantly increased (P<0.01) in T4, T5, T6. H₂S concentration had the significant influence on the activities of serum GSH-Px (P<0.05), SOD (P<0.01), CAT (P<0.01) and MDA content (P<0.05) at the 2nd day, and the activities of serum T-AOC (P<0.01), SOD (P<0.01), CAT (P<0.01), MDA content (P<0.01) at the 6th day. NH₃ concentration also had the significant influence on the activities of serum SOD (P<0.05), CAT (P<0.05) at the 2nd day, and the activities of serum SOD (P<0.01), CAT (P<0.01) at the 6th day. With the increase of time in climate chambers, the activities of serum SOD, CAT decreased. NH₃ and H₂S concentrations had the significant interaction effect on the activities of serum T-AOC (P<0.05), SOD (P<0.01), CAT (P<0.01), GSH-Px (P<0.01) at the 2nd day, and the activities of serum SOD (P<0.01), CAT (P<0.01) at the 6th day. It is concluded that NH₃ and H₂S could cause stress in goats and reduced the immune function and antioxidant capacity of goats. Furthermore, with the increase of NH₃ and H₂S concentrations and the prolongation of the action time, the degree of damage to the goats became worse.

中图分类号:

S827.46

赵天, 王国军, 彭孝坤, 张恩平. 氨气和硫化氢应激对肉羊免疫及抗氧化功能的影响[J]. 畜牧兽医学报, 2018, 49(10): 2191-2204.

ZHAO Tian, WANG Guo-jun, PENG Xiao-kun, ZHANG En-ping. Effects of Ammonia and Hydrogen Sulfide Stress on Immunity and Antioxidant Function of Goats[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(10): 2191-2204.

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参考文献

[1] 蔡丽媛.集约化羊舍的环境控制及热应激对山羊瘤胃发酵的影响[D].武汉:华中农业大学,2015.
CAI L Y.Environmental control of intensive goat buildings and the effects of heat stress on rumen fermentation of goats[D].Wuhan:Huazhong Agricultural University,2015.(in Chinese)
[2] 王金文.加强环境调控,实施肉羊健康养殖[C]//2013中国羊业进展.昌吉:中国畜牧业协会,2013:7.
WANG J W.Strengthen environmental regulation and implement healthy breeding of sheep[C]//2013 Sheep Industry in China.Changji:China Animal Husbandry Association,2013:7.(in Chinese)
[3] 王厚彬.环境因素对肉羊养殖的影响及其控制[J].现代畜牧科技,2017(4):32.
WANG H B.Influence of environmental factors on sheep breeding and its control[J].Modern Animal Husbandry Science & Technology,2017(4):32.(in Chinese)
[4] POKHAREL B B,DOS SANTOS V M,WOOD D,et al.Laying hens behave differently in artificially and naturally sourced ammoniated environments[J].Poult Sci,2017,96(12):4151-4157.
[5] PHILLIPS C J C,PINES M K,LATTER M,et al.Physiological and behavioral responses of sheep to gaseous ammonia[J].J Anim Sci,2012,90(5):1562-1569.
[6] PHILLIPS C J C,PINES M K,LATTER M,et al.The physiological and behavioral responses of steers to gaseous ammonia in simulated long-distance transport by ship[J].J Anim Sci,2010,88(11):3579-3589.
[7] YI B,CHEN L,SA R N,et al.High concentrations of atmospheric ammonia induce alterations of gene expression in the breast muscle of broilers (Gallus gallus) based on RNA-Seq[J].BMC Genomics,2016,17:598.
[8] DAVID B,MEJDELL C,MICHEL V,et al.Air quality in alternative housing systems may have an impact on laying hen welfare.Part Ⅱ-ammonia[J].Animals,2015,5(3):886-896.
[9] WEI F X,HU X F,SA R N,et al.Antioxidant capacity and meat quality of broilers exposed to different ambient humidity and ammonia concentrations[J].Genet Mol Res,2014,13(2):3117-3127.
[10] 高玉红,孙新胜,曹玉凤,等.密闭肉牛舍冬季有害气体的日变化和垂直分布规律研究[J].东北农业大学学报,2013,44(11):143-146.
GAO Y H,SUN X S,CAO Y F,et al.Daily variation and vertical distribution of harmful gases in enclosed houses for beef cattle[J].Journal of Northeast Agricultural University,2013,44(11):143-146.(in Chinese)
[11] 王亚男,冯曼,李宏双,等.坝上地区奶牛舍和犊牛舍冬季有害气体的检测[J].中国畜牧兽医,2016,43(6):1635-1640.
WANG Y N,FENG M,LI H S,et al.Investigation of harmful gases of dairy cow houses and calf houses in winter in Bashang Region[J].China Animal Husbandry & Veterinary Medicine,2016,43(6):1635-1640.(in Chinese)
[12] 李宏双,吴广军,张久德,等.河北省北方山区不同建筑类型奶牛舍和犊牛舍冬季有害气体的检测[J].中国畜牧杂志,2015,51(20):81-85.
LI H S,WU G J,ZHANG J D,et al.Investigation of noxious gas in different styles of dairy cattle houses and calf houses in winter in northern mountainous area of Hebei Province[J].Chinese Journal of Animal Science,2015,51(20):81-85.(in Chinese)
[13] 赵育国,史彬林,崔玉铭,等.北方农牧交错区奶牛场舍内外有害气体的季节性变化[C]//通辽:畜牧业环境、生态、安全生产与管理——2010年家畜环境与生态学术研讨会论文集.2010:11-14.
ZHAO Y G,SHI B L,CUI Y M,et al.Seasonal change of noxious gas inside and outside the dairy house in farming-pastoral transition area of northern China[C]//The 2010 Symposium on Environmental and Ecological Livestock.2010:11-14.(in Chinese)
[14] 丁莹,黎明,岳远西,等.内蒙古规模化羊场不同类型羊舍空气质量评价[J].家畜生态学报,2017,38(2):58-63,96.
DING Y,LI M,YUE Y X,et al.Assessment of air quality in different types of sheep houses in Inner Mongolian sheep farm[J].Acta Ecologiae Animalis Domastici,2017,38(2):58-63,96.(in Chinese)
[15] 赵有璋.国内外养羊业发展趋势、问题和对策[J].现代畜牧兽医,2015(9):63-68.
ZHAO Y Z.Development tendency,problems and countermeasures on sheep industry in domestic and overseas[J].Modern Journal of Animal Husbandry and Veterinary Medicine,2015(9):63-68.(in Chinese)
[16] 中华人民共和国农业部.中华人民共和国农业行业标准畜禽场环境质量标准NY/T388-1999[J].养猪,2005(1):42-43.
Ministry of Agriculture of the PRC.Environmental quality standard for the livestock and poultry farm NY/T388-1999[J].Swine Production,2005(1):42-43.(in Chinese)
[17] 张克烽,张子平,陈芸,等.动物抗氧化系统中主要抗氧化酶基因的研究进展[J].动物学杂志,2007,42(2):153-160.
ZHANG K F,ZHANG Z P,CHEN Y,et al.Antioxidant defense system in animals[J].Chinese Journal of Zoology,2007,42(2):153-160.(in Chinese)
[18] 杨汉春.动物免疫学[M].2版.北京:中国农业大学出版社,2003.
YANG H C.Animal immunology[M].2nd ed.Beijing:China Agricultural University Press,2003.(in Chinese)
[19] 贺喜.日粮油脂对不同免疫状态肉鸡免疫调控作用及其信号传导相关机理[D].北京:中国农业大学,2007.
HE X.Effects of dietray oils on immune function and related signal transduction in chickens under different immune status[D].Beijing:China Agricultural University,2007.(in Chinese)
[20] 刁华杰,冯京海,王雪洁,等.日循环高温对蛋鸡抗氧化能力及免疫机能的影响[J].畜牧兽医学报,2017,48(6):1044-1053.
DIAO H J,FENG J H,WANG X J,et al.Effect of cyclic high temperature on antioxidant capacity and immune function of laying hens[J].Acta Veterinaria et Zootechnica Sinica,2017,48(6):1044-1053.(in Chinese)
[21] 孟庆平.不同硫化氢浓度对肉仔鸡生长性能、免疫功能和肉质的影响[D].杭州:浙江大学,2009.
MENG Q P.Effects of atmospheric hydrogen sulfide concentration on growth,immunity and meat quality[D].Hangzhou:Zhejiang University,2009.(in Chinese)
[22] 郭晓飞,陈俊,张子军,等.不同羊舍类型及饲养密度对山羊血清生化指标的影响[J].安徽农业大学学报,2014,41(4):585-591.
GUO X F,CHEN J,ZHANG Z J,et al.Effects of different sheds and stocking densities on goat serum biochemical indexes[J].Journal of Anhui Agricultural University,2014,41(4):585-591.(in Chinese)
[23] 姚中磊.不同氨气浓度对肉仔鸡生长、免疫和血液生理生化指标的影响[D].杭州:浙江大学,2008.
YAO Z L.Effects of different ammonia concentrations on growth,immunity and physiological-biochemical parameters in broiler chickens[D].Hangzhou:Zhejiang University,2008(in Chinese)
[24] 宋弋,王忠,姚中磊,等.慢性氨气暴露对肉鸡NK细胞杀伤活性和血清新城疫抗体效价和溶菌酶的影响[J].中国畜牧杂志,2009,45(7):47-50.
SONG Y,WANG Z,YAO Z L,et al.Effects of chronic ammonia exposure on natural killer cell activity,newcastle disease antibody titer and serum lysozyme levels of broiler[J].Chinese Journal of Animal Science,2009,45(7):47-50.(in Chinese)
[25] WANG Y M,MENG Q P,GUO Y M,et al.Effect of atmospheric ammonia on growth performance and immunological response of broiler chickens[J].J Anim Vet Adv,2010,9(22):2802-2806.
[26] WU Y N,YAN F F,HU J Y,et al.The effect of chronic ammonia exposure on acute-phase proteins,immunoglobulin,and cytokines in laying hens[J].Poult Sci,2017,96(6):1524-1530.
[27] WEI F X,HU X F,XU B,et al.Ammonia concentration and relative humidity in poultry houses affect the immune response of broilers[J].Genet Mol Res,2015,14(2):3160-3169.
[28] 高艳霞,仁瑞清,李建国,等.断奶应激对犊牛血液生化、免疫和内分泌的影响[J].中国兽医学报,2014,34(8):1294-1299,1303.
GAO Y X,REN R Q,LI J G,et al.Effects of weaning stress on blood biochemical indicators,immunity and endocrine of dairy calves[J].Chinese Journal of Veterinary Science,2014,34(8):1294-1299,1303.(in Chinese)
[29] 张洪英,鲍恩东.热应激对雏鸡自然杀伤(NK)细胞活性和T淋巴细胞转化率的影响[J].南京农业大学学报,2000,23(4):85-88.
ZHANG H Y,BAO E D.Influences of heat shock on natural killer (NK)cell activities and lymphocyte proliferation rates of chicken[J].Journal of Nanjing Agricultural University,2000,23(4):85-88.(in Chinese)
[30] KELLEY K W.Stress and immune function:A bibliographic review[J].Ann Rech Vet,1980,11(4):445-478.
[31] YOUNG R A.Stress proteins and immunology[J].Annu Rev Immunol,1990,8:401-420.
[32] 魏凤仙,胡骁飞,张敏红,等.相对湿度和氨气应激对肉仔鸡血氨水平及细胞因子含量的影响[J].动物营养学报,2013,25(10):2246-2253.
WEI F X,HU X F,ZHANG M H,et al.Effects of relative humidity and ammonia stress on plasma ammonia level and cytokine contents of broilers[J].Chinese Journal of Animal Nutrition,2013,25(10):2246-2253.(in Chinese)
[33] KARAKURUM M,SHREENIWAS R,CHEN J,et al.Hypoxic induction of interleukin-8 gene expression in human endothelial cells[J].J Clin Invest,1994,93(4):1564-1570.
[34] HARTMANN G,TSCHÖP M,FISCHER R,et al.High altitude increases circulating interleukin-6,interleukin-1 receptor antagonist and C-reactive protein[J].Cytokine,2000,12(3):246-252.
[35] APPELS A,BÄR F W,BÄR J,et al.Inflammation,depressive symptomatology,and coronary artery disease[J].Psychosom Med,2000,62(5):601-605.
[36] 赵克然,杨毅军,曹道俊.氧自由基与临床[M].北京:中国医药科技出版社,2000.
ZHAO K R,YANG Y J,CAO D J.Oxygen free radicals and clinical[M].Beijing:China Medical Science Press,2000(in Chinese)
[37] 刘红,杨宏波,朱建明,等.半胱胺盐酸盐对奶牛营养物质消化率、血清生化及抗氧化指标的影响[J].畜牧兽医学报,2015,46(3):416-423.
LIU H,YANG H B,ZHU J M,et al.Effects of cysteamine hydrochloride on apparent nutrient digestibility,serum biochemical and antioxidant indices of lactating dairy cows[J].Acta Veterinaria et Zootechnica Sinica,2015,46(3):416-423.(in Chinese)
[38] 闵育娜,李华磊,刘少凯,等.酒糟对氧化应激肉鸡肉品质和抗氧化能力的影响[J].西北农业学报,2014,23(12):43-50.
MIN Y N,LI H L,LIU S K,et al.Effects of dietary DDGS on meat quality and antioxidant capacity of oxidative stress broiler chickens[J].Acta Agriculturae Boreali-occidentalis Sinica,2014,23(12):43-50.(in Chinese)
[39] 高晶.应激对肉鸡脂质过氧化状态及肉质的影响[D].泰安:山东农业大学,2008.
GAO J.Effect of stress on lipid peroxidation and meat quality of broilers[D].Tai'an:Shandong Agricultural University,2008.(in Chinese)
[40] 胡国良,花象柏,周吉薇,等.产蛋鸡热应激时血液中与体内自由基有关的生化物质含量变化的分析[J].养禽与禽病防治,1997(1):6-7.
HU G L,HUA X B,ZHOU J W,et al.Analysis of changes of biochemical substances concerning free radicals in blood of heat stress laying hens[J].Poultry Husbandry and Disease Control,1997(1):6-7.(in Chinese)
[41] 唐燕红,王剑,李金钢,等.低氧应激下甘肃鼢鼠与SD大鼠心脏抗氧化酶和ATP酶活性的比较[J].兽类学报,2013,33(2):178-185.
TANG Y H,WANG J,LI J G,et al.Comparision of antioxidase and ATPase activity in heart between Myospalax cansus and Rattus norvegicus under acute hypoxia[J].Acta Theriologica Sinica,2013,33(2):178-185.(in Chinese)
[42] 邢焕,栾素军,孙永波,等.舍内不同氨气浓度对肉鸡抗氧化性能及肉品质的影响[J].中国农业科学,2015,48(21):4347-4357.
XING H,LUAN S J,SUN Y B,et al.Effect of ammonia in broiler houses on the antioxidant activity and meat quality of broiler[J].Scientia Agricultura Sinica,2015,48(21):4347-4357.(in Chinese)
[43] 王剑.急性低氧下甘肃鼢鼠心脏抗氧化酶及ATP酶活性研究[D].西安:陕西师范大学,2010.
WANG J.Study on antioxidant enzymes and ATPase of Gansu zokor's myocardial in acute hypoxia[D].Xi'an:Shaanxi Normal University,2010.(in Chinese)