郁金散对大肠湿热证大鼠血清免疫球蛋白与抗氧化相关因子的调节作用

doi:10.11843/j.issn.0366-6964.2018.09.026

摘要/Abstract

摘要：

本试验旨在阐明郁金散对大肠湿热证大鼠血清免疫球蛋白与抗氧化相关因子的调节作用。选取60只Wistar大鼠，随机分为正常对照组、大肠湿热证模型组、自愈组以及郁金散高、中、低剂量治疗组，共6组，每组10只。通过模拟大肠湿热证发病条件，饮食中采用高糖高脂饮食、饥饱失常、灌服白酒，设置高温高湿环境，再腹腔注射生物因子大肠杆菌，以建立大肠湿热证大鼠模型。此后分别用高、中、低剂量郁金散治疗5 d。然后检测血清免疫球蛋白IgA、IgG、IgM水平，并采集脾、胸腺进行组织病理学观察；同时检测血清及肝组织脂质过氧化物（LPO）、丙二醛（MDA）、还原型谷胱甘肽（GSH）、一氧化氮（NO）水平。结果显示：与正常对照组相比，模型组血清IgA、IgG、IgM含量极显著升高（P<0.01）；与自愈组相比，各剂量郁金散治疗组有不同程度的回调，其中以高剂量组回调最明显，高剂量组中IgA和IgM含量显著降低（P<0.05），IgG含量极显著降低（P<0.01）。病理切片显示大肠湿热证模型大鼠脾和胸腺组织形态结构紊乱，脾淤血，胸腺严重萎缩；郁金散高剂量组脾结构趋于正常，胸腺萎缩程度降低。与正常对照组相比，模型组大鼠血清和肝组织中MDA和LPO含量极显著升高（P<0.01），GSH和NO含量极显著降低（P<0.01）；与自愈组相比，各剂量郁金散治疗组有不同程度的回调，其中以高剂量组回调最明显，MDA和LPO含量显著或极显著降低（P<0.05或P<0.01），GSH和NO含量显著或极显著升高（P<0.05或P<0.01）。大肠湿热证模型大鼠机体免疫功能异常、氧化与抗氧化平衡紊乱，郁金散可通过回调大肠湿热证机体的血清免疫球蛋白水平，改善免疫器官脾和胸腺的结构，调节机体的氧化和抗氧化平衡关系而治疗大肠湿热证，其中以高剂量组治疗效果最好。

Abstract:

This study was conducted to elucidate the regulation of Yujin Powder (YJP) on serum immunoglobulin and antioxidance-related factors of Large Intestine Dampness-heat Syndrome (LIDHS) rat. Sixty Wistar rats were randomly divided into 6 groups with 10 rats in each group:normal control group, LIDHS model group, self-healing group, high, middle and low dose of YJP groups. The rat model of LIDHS was established by such complex factors as high-sugar and high-fat diet, improper diet, drinking, high temperature and humidity environment and intrap-eritoneal injection of Escherichia coli, which imitated the inducing conditions of LIDHS. After the model being successfully established, the rats were treated for 5 days with high, middle and low dose of YJP, respectively. And then levels of serum immunoglobulin IgA, IgG, IgM were detected, the spleen and thymus were collected for histopathological observation, and the lipid peroxide (LPO), malondialdehyde (MDA), reduced glutathione (GSH), and oxide nitrogen (NO) levels in serum and liver tissue homogenate were detected. The results showed that compared with the normal control group, the serum levels of IgA, IgG and IgM in the model group were significantly increased (P<0.01). In comparison with the self-healing group, they were all reversed to different degrees after different doses of YJP administration, and the most obviously in the high-dose group. In high dose group, the content of IgA and IgM were significantly decreased (P<0.05) and IgG was extremely significantly decreased (P<0.01). The pathological observation showed that the splenic and thymic morphological structure of model group rat disorder, spleen congestion, and severe thymus atrophy. In the high-dose group of YJP, the spleen structure tends to be normal, and the degree of thymus atrophy was reduced. Compared with the normal control group, the content of MDA and LPO in the serum and liver tissues in the model group were extremely significantly increased (P<0.01), and the content of GSH and NO were extremely significantly decreased (P<0.01). Compared with the self-healing group, they were all reversed to different degrees after treatment with different doses of YJP administration, and the most obviously in the high-dose group. In high dose group, the content of MDA and LPO were significantly or extremely significantly decreased (P<0.05 or P<0.01), and the GSH and NO content were significantly or extremely significantly increased (P<0.05 or P<0.01). There were abnormal immune function and disturbance of oxidation and antioxidant balance in LIDHS model rats. YJP can treat LIDHS through reducing the serum immunoglobulin levels, improving the structure of spleen and thymus and regulating the balance of oxidation and antioxidation. And the effect of high dose was the best.

中图分类号:

S853.3

张亚辉, 姚万玲, 文艳巧, 华永丽, 纪鹏, 魏彦明. 郁金散对大肠湿热证大鼠血清免疫球蛋白与抗氧化相关因子的调节作用[J]. 畜牧兽医学报, 2018, 49(9): 2044-2053.

ZHANG Ya-hui, YAO Wan-ling, WEN Yan-qiao, HUA Yong-li, JI Peng, WEI Yan-ming. Regulation of Yujin Powder on Serum Immunoglobulin and Antioxidant Related Factors of Large Intestine Dampness-heat Syndrome Rat[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(9): 2044-2053.

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