新疆野生一枝蒿粗多糖对流感疫苗小鼠免疫增强效果的分析

doi:10.11843/j.issn.0366-6964.2016.10.018

畜牧兽医学报 ›› 2016, Vol. 47 ›› Issue (10): 2089-2097.doi: 10.11843/j.issn.0366-6964.2016.10.018

新疆野生一枝蒿粗多糖对流感疫苗小鼠免疫增强效果的分析

张爱莲^*，王丹阳，赵淑述，赵兵，张富春

（新疆大学生命科学与技术学院新疆生物资源与基因工程重点实验室，乌鲁木齐 830046）

收稿日期:2016-05-16 出版日期:2016-10-23 发布日期:2016-10-23
通讯作者: 张爱莲，副教授，E-mail：xjzal@163.com
作者简介:张爱莲(1968-)，女，山东东明人，副教授，博士，主要从事疫苗佐剂方面的研究，Tel：0991-5523400
基金资助:
自治区自然科学基金（201404061219）

The Analysis of Immune Enhancement Effect of Xinjiang Wild Artemisia rupestris L.Crude Polysaccharides on Influenza Viruses Vaccines in Mice

ZHANG Ai-lian^*，WANG Dan-yang，ZHAO Shu-shu，ZHAO Bing，ZHANG Fu-chun

(Xinjiang Key Laboratory of Biological Resources and Genetic Engineering，College of Life Science and Technology，Xinjiang University，Urumqi 830046，China)

Received:2016-05-16 Online:2016-10-23 Published:2016-10-23

摘要/Abstract

摘要：

用新疆野生一枝蒿粗多糖（wild Artemisia rupestris L.crude polysaccharides，WARCP）通过肌肉注射免疫小鼠，研究其作为流感病毒疫苗（influenza virus vaccine，IVV）的佐剂效果和节约流感疫苗抗原用量的作用。WARCP与不同剂量（0.05、0.1、0.5 μg）的IVV配伍，肌肉注射免疫小鼠，观察小鼠的生长状态，通过ELISA法检测血清中抗体IgG及IgG₁、IgG_2a的水平；MTT法检测脾淋巴细胞的增殖；流式细胞术检测细胞因子CD4⁺IL-4和CD8⁺IFN-γ以及CD4⁺CD25⁺Foxp3⁺Treg细胞的表达水平。结果表明WARCP对小鼠的生长状态没有显著的影响(P>0.05)；WARCP能够显著提高不同剂量IVV特异性IgG及其IgG₁、IgG_2a的抗体水平(P<0.05)；增强淋巴细胞的增殖（P<0.05）；显著提高IL-4和IFN-γ的分泌水平(P<0.05)；降低Treg细胞的表达水平（P<0.05）；尤其可以提高低剂量IVV的体液和细胞免疫反应，至少减少抗原用量91%。因此，WARCP作为IVV的佐剂通过降低Treg细胞的水平，显著增强了IVV的免疫效果，尤其是Th1型的免疫反应，能够很好地节约抗原用量，具有良好的安全性。

Abstract:

The immune effects of Xinjiang Wild Artemisia rupestris L.crude polysaccharides (WARCP) as an adjuvant and sparing antigen on different doses of influenza virus vaccine (IVV) were explored.ICR mice were intramuscularly immunized respectively with different doses (0.05，0.1，0.5 μg) of the IVV alone or co-administered with 300 μg WARCP at 0 d and 14 d.The growth state of mice were observed，antibody level of IgG and IgG₁，IgG_2a in serum were tested by ELISA；splenocyte proliferations were tested by MTT；The expression levels of CD4⁺IL-4，CD8⁺IFN-γ and CD4⁺CD25⁺Foxp3⁺Treg cells were tested by flow cytometry.The results showed that no significant differences in the body weight were observed between mice from different groups (P>0.05)；WARCP can significantly improve the antibody level of influenza-specific IgG and IgG₁，IgG_2a(P<0.05)；WARCP can significantly promote lymphocyte proliferation，the secretion level of IL-4 and IFN-γ (P<0.05),and significantly reduce the level of expression of Treg cells (P<0.05)；In particularly，it can enhance the humoral and cellular immunity responses of low-dose IVV,indicating an at least 91% reduction in vaccine dosage by adding WARCP as adjuvant.These results indicated that adding WARCP to IVV enhanced the immune efficacy of IVV by reducing the level of Treg cells，significantly promote Th1-type immune response particularly，it can be an adjuvant with the advantages of high safety and dose-sparing.

中图分类号:

S852.52

张爱莲，王丹阳，赵淑述，赵兵，张富春. 新疆野生一枝蒿粗多糖对流感疫苗小鼠免疫增强效果的分析[J]. 畜牧兽医学报, 2016, 47(10): 2089-2097.

ZHANG Ai-lian，WANG Dan-yang，ZHAO Shu-shu，ZHAO Bing，ZHANG Fu-chun. The Analysis of Immune Enhancement Effect of Xinjiang Wild Artemisia rupestris L.Crude Polysaccharides on Influenza Viruses Vaccines in Mice[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(10): 2089-2097.

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新疆野生一枝蒿粗多糖对流感疫苗小鼠免疫增强效果的分析

The Analysis of Immune Enhancement Effect of Xinjiang Wild Artemisia rupestris L.Crude Polysaccharides on Influenza Viruses Vaccines in Mice

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