牦牛角性状候选基因的筛选

doi:10.11843/j.issn.0366-6964.2016.06.009

畜牧兽医学报 ›› 2016, Vol. 47 ›› Issue (6): 1147-1153.doi: 10.11843/j.issn.0366-6964.2016.06.009

牦牛角性状候选基因的筛选

佘平昌^1，2，吴晓云^1，2，梁春年^1，2，禇敏^1，2，丁学智^1，2，阎萍^1，2*

（1.中国农业科学院兰州畜牧与兽药研究所，兰州 730050； 2.甘肃省牦牛繁育工程重点实验室，兰州 730050）

收稿日期:2015-11-02 出版日期:2016-06-23 发布日期:2016-06-23
通讯作者: 阎萍，研究员，博士生导师，主要从事动物遗传育种与繁殖研究，E-mail：pingyanlz@163.com
作者简介:佘平昌（1989-），男，云南普洱人，硕士生，主要从事家畜分子生物学研究，E-mail：sheemail@163.com
基金资助:
牦牛遗传资源与育种(CAAS-ASTIP-2014-LIHPS-01)；科技部科技支撑计划(2012BAD13B05)

Screening of Candidate Genes for Horn Trait of Yak

SHE Ping-chang^1，2，WU Xiao-yun^1，2，LIANG Chun-nian^1，2，CHU Min^1，2，DING Xue-zhi^1，2，YAN Ping^1，2*

（1.Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences，Lanzhou 730050，China；2. Key Laboratory of Yak Breeding Engineering of Gansu Province， Lanzhou 730050，China）

Received:2015-11-02 Online:2016-06-23 Published:2016-06-23

摘要/Abstract

摘要：

本研究旨在筛选牦牛角性状候选基因，为角发育机制的研究和无角牦牛的培育提供科学依据。采集牦牛角发育初期胎龄90 d的有角和无角牛的角基及额部皮肤组织，利用实时荧光定量PCR技术（qRT-PCR），比较牛角性状候选基因OLIG1、OLIG2、IFNAR1、IFNAR2、C1H21orf62、GCFC1、IFNGR2、SYNJ1、IL10RB、GART、RXFP2、FOXL2、TWIST1、TWIST2、ZEB2、E-cadherin和P-cadherin mRNA在角基间及皮肤间的相对表达量。结果显示，候选基因OLIG1、IFNAR1、C1H21orf62、SYNJ1、IL10RB、GART、RXFP2、TWIST2和E-cadherin在有角和无角牦牛角基间和皮肤间转录量差异不显著（P>0.05）。 IFNGR2、FOXL2、TWIST1和P-cadherin在有角和无角牦牛角基间转录量差异显著（P<0.05）。IFNAR2和GCFC1在有角和无角牦牛角基间和皮肤间转录量差异都显著（P<0.05）。OLIG2和ZEB2在有角和无角牦牛角基间转录量差异极显著（P<0.01）。推测OLIG2和FOXL2可能是牛角性状的关键候选基因，同时ZEB2、TWIST1和IFNGR2基因可能对牛角的形成具有重要作用，而P-cadherin基因可能参与了牛角的发育。

Abstract:

This study screened the candidate genes of horn trait which provides scientific basis for the horn developmental mechanism and the polled yak breeding.The horn bud and forehead skin tissue from horn and polled yaks with 90 d gestational age were collected，and the mRNA different expression of OLIG1,OLIG2,IFNAR1,IFNAR2,C1H21orf62,GCFC1,IFNGR2,SYNJ1,IL10RB,GART,RXFP2,FOXL2,TWIST1,TWIST2,ZEB2,E-cadherin,P-cadherin genes were analyzed using real-time quantitative PCR（qRT-PCR）.The results showed that expressions of OLIG1,IFNAR1,C1H21orf62,SYNJ1,IL10RB,GART,RXFP2,TWIST2 and E-cadheringenes showed no significant differences in horn and skin between horned and polled yaks （P>0.05）.There were significant difference at transcript levels of IFNGR2，FOXL2，TWIST1 and P-cadherin genes in horn buds between horned and polled yaks（P<0.05）.IFNAR2 and GCFC1 had significant transcription difference in the expression of horn bud and skin between horned and polled yaks （P<0.05）.OLIG2 and ZEB2 had highly significant transcription difference in horn bud expression between horned and polled yaks （P<0.01）.We concluded that OLIG2 and FOXL2 might be the key candidate genes.ZEB2，TWIST1 and IFNGR2 might also involve in the formation of horn. P-cadherin might participate in the development of horn.

中图分类号:

佘平昌，吴晓云，梁春年，禇敏，丁学智，阎萍. 牦牛角性状候选基因的筛选[J]. 畜牧兽医学报, 2016, 47(6): 1147-1153.

SHE Ping-chang，WU Xiao-yun，LIANG Chun-nian，CHU Min，DING Xue-zhi，YAN Ping. Screening of Candidate Genes for Horn Trait of Yak[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(6): 1147-1153.

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牦牛角性状候选基因的筛选

Screening of Candidate Genes for Horn Trait of Yak

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