具有E3泛素连接酶功能的痘病毒蛋白质

doi:10.11843/j.issn.0366-6964.2015.12.002

摘要/Abstract

摘要：

泛素化是蛋白质的翻译后共价修饰过程，可以调节蛋白质的稳定性，涉及多种生理功能。痘病毒在进化过程中形成了扰乱和操纵宿主细胞泛素系统的多种机制，包括编码多种能干扰宿主泛素系统的病毒蛋白质。近年的研究表明痘病毒编码许多具有泛素连接酶活性的蛋白质，如膜相关RING-CH（MARCH）结构域蛋白、p28/Really Interesting New Gene（RING）finger蛋白、锚蛋白重复序列/F-box蛋白、Bric-a-Brac Tramtrack Broad complex（BTB）/Kelch蛋白及APC家族蛋白等。作者在本文着重阐述了痘病毒编码的E3泛素连接酶功能蛋白的研究概况。

Abstract:

Ubiquitylation is a covalent post-translational modification that regulates protein stability and is involved in many biological functions.Many poxviruses have evolved multiple mechanisms to perturb the cell ubiquitin system and manipulate it to their own benefit.Such as the poxvirus family encode ubiquitin ligases with intrinsic activity，including the membrane-associated RING-CH(MARCH) domain，p28/Really Interesting New Gene(RING) finger，ankyrin-repeat/F-box and Broad-complex，Tramtrack and Bric-a-Brac(BTB)/Kelch subgroups of the E3 Ub ligase superfamily.Here we describe and discuss the proteins with E3 ubiquitin ligase function encoded by poxvirus.

中图分类号:

陈轶霞，邵忠伟，李贵华，王聪. 具有E3泛素连接酶功能的痘病毒蛋白质[J]. 畜牧兽医学报, 2015, 46(12): 2127-2134.

CHEN Yi-xia，SHAO Zhong-wei，LI Gui-hua，WANG Cong. Poxvirus Encode Proteins with E3 Ubiquitin Ligase Function[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(12): 2127-2134.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.xmsyxb.com/CN/10.11843/j.issn.0366-6964.2015.12.002

https://www.xmsyxb.com/CN/Y2015/V46/I12/2127

参考文献

［1］MOSS B.Poxviridae：the viruses and their replicaton［M］//Fields Virology.5th edn.KNIPE D M，HOWLEY P M，GRIFFIN D E，et al.Philadelphia，PA：Lippincott Williams & Wilkins，2007：2905-2946.
［2］RANDOW F，LEHNER P J.Viral avoidance and exploitation of the ubiquitin system［J］.Nat Cell Biol，2009，11(5)：527-534.
［3］GUSTIN J K，MOSES A V，FRÜH K，et al.Viral takeover of the host ubiquitin system［J］.Front Microbiol，2011，2：161.
［4］BAUMGÄRTEL V，IVANCHENKO S，DUPONT A，et al.Live-cell visualization of dynamics of HIV budding site interactions with an ESCRT component［J］.Nat Cell Biol，2011，13(4)：469-474.
［5］MORITA E，SANDRIN V，MCCULLOUGH J，et al.ESCRT-III protein requirements for HIV-1 budding［J］.Cell Host Microbe，2011，9(3)：235-242.
［6］LIAO T L，WU C Y，SU W C，et al.Ubiquitination and deubiquitination of NP protein regulates influenza A virus RNA replication［J］.EMBO J，2010，29(22)：3879-3890.
［7］TAYLOR R T，LUBICK K J，ROBERTSON S J，et al.TRIM79α，an interferon-stimulated gene product，restricts tick-borne encephalitis virus replication by degrading the viral RNA polymerase［J］.Cell Host Microbe，2011，10(3)：185-196.
［8］KAMIO M，YOSHIDA T，OGATA H，et al.SOCS1［corrected］inhibits HPV-E7-mediated transformation by inducing degradation of E7 protein［J］.Oncogene，2004，23(17)：3107-3115.
［9］BARRY M，VAN BUUREN N，BURLES K，et al.Poxvirus exploitation of the ubiquitin-proteasome system［J］.Viruses，2010，2(10)：2356-2380.
［10］SHCHELKUNOV S N.Interaction of orthopoxviruses with the cellular ubiquitin-ligase system［J］.Virus Genes，2010，41(3)：309-318.
［11］MANSUR D S，MALUQUER DE MOTES C，UNTERHOLZNER L，et al.Poxvirus targeting of E3 ligase β-TrCP by molecular mimicry：a mechanism to inhibit NF-κB activation and promote immune evasion and virulence［J］. PLoS Pathog，2013，9(2)：e1003183.
［12］MO M，FLEMING S B，MERCER A A.Cell cycle deregulation by a poxvirus partial mimic of anaphase-promoting complex subunit 11［J］.Proc Natl Acad Sci U S A，2009，106(46)：19527-19532.
［13］MERCER J，SNIJDER B，SACHER R，et al.RNAi screening reveals proteasome-and cullin3-dependent stages in vaccinia virus infection［J］.Cell Rep，2012，2(4)：1036-1047.
［14］KOMANDER D，RAPE M.The ubiquitin code［J］.Annu Rev Biochem，2012，81：203-229.
［15］OHMURA-HOSHINO M，GOTO E，MATSUKI Y，et al.A novel family of membrane-bound E3 ubiquitin ligases［J］.J Biochem，2006，140(2)：147-154.
［16］GUERIN J L，GELFI J，BOULLIER S，et al.Myxoma virus leukemia-associated protein is responsible for major histocompatibility complex class I and Fas-CD95 down-regulation and defines scrapins，a new group of surface cellular receptor abductor proteins［J］.J Virol，2002，76(6)：2912-2923.
［17］BARTEE E，MCCORMACK A，FRÜH K.Quantitative membrane proteomics reveals new cellular targets of viral immune modulators［J］.PLoS Pathog，2002，2(10)：e107.
［18］SENKEVICH T G，WOLFFE E J，BULLER R M.Ectromelia virus RING finger protein is localized in virus factories and is required for virus replication in macrophages［J］.J Virol，1995，69(7)：4103-4111.
［19］NERENBERG B T，TAYLOR J，BARTEE E，et al.The poxviral RING protein p28 is a ubiquitin ligase that targets ubiquitin to viral replication factories［J］.J Virol，2005，79(1)：597-601.
［20］MOTTET K，BAREISS B，MILNE C D，et al.The poxvirus encoded ubiquitin ligase，p28，is regulated by proteasomal degradation and autoubiquitination［J］.Virology，2014，468-470：363-378.
［21］BAREISS B，BARRY M.Fowlpox virus encodes two p28-like ubiquitin ligases that are expressed early and late during infection［J］.Virology，2014，462-463：60-70.
［22］JOHNSTON J B，WANG G，BARRETT J W，et al.Myxoma virus M-T5 protects infected cells from the stress of cell cycle arrest through its interaction with host cell cullin-1［J］.J Virol，2005，79(16)：10750-10763.
［23］WERDEN S J，LANCHBURY J，SHATTUCK D，et al.The myxoma virus m-t5 ankyrin repeat host range protein is a novel adaptor that coordinately links the cellular signaling pathways mediated by Akt and Skp1 in virus-infected cells［J］.J Virol，2009，83(23)：12068-12083.
［24］BLANIÉ S，MORTIER J，DELVERDIER M，et al.M148R and M149R are two virulence factors for myxoma virus pathogenesis in the European rabbit［J］.Vet Res，2009，40(1)：11.
［25］SPERLING K M，SCHWANTES A，SCHNIERLE B S，et al.The highly conserved orthopoxvirus 68k ankyrin-like protein is part of a cellular SCF ubiquitin ligase complex［J］.Virology，2008，374(2)：234-239.
［26］SONNBERG S，SEET B T，PAWSON T，et al.Poxvirus ankyrin repeat proteins are a unique class of F-box proteins that associate with cellular SCF1 ubiquitin ligase complexes［J］.Proc Natl Acad Sci U S A，2008，105(31)：10955-10960.
［27］BURLES K，VAN BUUREN N，BARRY M.Ectromelia virus encodes a family of Ankyrin/F-box proteins that regulate NFκB［J］.Virology，2014，468-470：351-362.
［28］WILTON B A，CAMPBELL S，VAN BUUREN N，et al.Ectromelia virus BTB/kelch proteins，EVM150 and EVM167，interact with cullin-3-based ubiquitin ligases［J］.Virology，2008，374(1)：82-99.
［29］KOCHNEVA G，KOLOSOVA I，MAKSYUTOVA T，et al.Effects of deletions of kelch-like genes on cowpox virus biological properties［J］.Arch Virol，2005，150(9)：1857-1870.
［30］ZHANG L，VILLA N Y，MCFADDEN G.Interplay between poxviruses and the cellular ubiquitin/ubiquitin-like pathways［J］.FEBS Lett，2009，583(4)：607-614.
［31］MANSOURI M，BARTEE E，GOUVEIA K，et al.The PHD/LAP-domain protein M153R of myxomavirus is a ubiquitin ligase that induces the rapid internalization and lysosomal destruction of CD4［J］.J Virol，2003，77(2)：1427-1440.
［32］UPTON C，SCHIFF L，RICE S A，et al.A poxvirus protein with a RING finger motif binds zinc and localizes in virus factories［J］.J Virol，1994，68(7)：4186-4195.
［33］HUANG J，HUANG Q，ZHOU X，et al.The poxvirus p28 virulence factor is an E3 ubiquitin ligase［J］.J Biol Chem，2004，279(52)：54110-54116.
［34］IYER L M，KOONIN E V，ARAVIND L.Extensive domain shuffling in transcription regulators of DNA viruses and implications for the origin of fungal APSES transcription factors［J］.Genome Biol，2002，3(3)：RESEARCH0012.
［35］BRICK D J，BURKE R D，SCHIFF L，et al.Shope fibroma virus RING finger protein N1R binds DNA and inhibits apoptosis［J］.Virology，1998，249(1)：42-51.
［36］TULMAN E R，AFONSO C L，LU Z，et al.The genome of canarypox virus［J］.J Virol，2004，78(1)：353-366.
［37］BRICK D J，BURKE R D，MINKLEY A A，et al.Ectromelia virus virulence factor p28 acts upstream of caspase-3 in response to UV light-induced apoptosis［J］.J Gen Virol，2000，81(Pt4)：1087-1097.
［38］PESIN J A，ORR-WEAVER T L.Regulation of APC/C activators in mitosis and meiosis［J］.Annu Rev Cell Dev Biol，2008，24：475-499.
［39］TANG Z，LI B，BHARADWAJ R，et al.APC2 Cullin protein and APC11 RING protein comprise the minimal ubiquitin ligase module of the anaphase-promoting complex［J］.Mo Biol Cell，2001，12(12)：3839-3851.
［40］AFONSO C L，TULMAN E R，DELHON G，et al.Genome of crocodilepox virus［J］.J Virol，2006，80(10)：4978-4991.
［41］SENKEVICH T G，KOONIN E V，BUGERT J J，et al.The genome of molluscum contagiosum virus：analysis and comparison with other poxviruses［J］.Virology，1997，233(1)：19-42.
［42］HERBERT M H，SQUIRE C J，MERCER A A.Poxviral ankyrin proteins［J］.Viruses，2015，7(2)：709-738.
［43］LAMB S A，RAHMAN M M，MCFADDEN G.Recombinant myxoma virus lacking all poxvirus ankyrin-repeat proteins stimulates multiple cellular anti-viral pathways and exhibits a severe decrease in virulence［J］.Virology，2014，464-465：134-145.
［44］BLANIÉ S，GELFI J，BERTAGNOLI S，et al.MNF，an ankyrin repeat protein of myxoma virus，is part of a native cellular SCF complex during viral infection［J］.Virol J，2010，7：56.
［45］CHANG S J，HSIAO J C，SONNBER G，et al.Poxvirus host range protein CP77 contains an F-box-like domain that is necessary to suppress NF-kappaB activation by tumor necrosis factor alpha but is independent of its host range function［J］.J Virol，2009，83(9)：4140-4152.
［46］MOHAMED M R，RAHMAN M M，LANCHBURY J S，et al.Proteomic screening of variola virus reveals a unique NF-kappaB inhibitor that is highly conserved among pathogenic orthopoxviruses［J］.Proc Natl Acad Sci U S A，2009，106(22)：9045-9050.
［47］MOHAMED M R，RAHMAN M M，RICE A，et al.Cowpox virus expresses a novel ankyrin repeat NF-kappaB inhibitor that controls inflammatory cell influx into virus-infected tissues and is critical for virus pathogenesis［J］.J Virol，2009，83(18)：9223-9236.
［48］STOGIOS P J，DOWNS G S，JAUHAL J J，et al.Sequence and structural analysis of BTB domain proteins［J］.Genome Biol，2005，6(10)：R82.
［49］KOTWAL G J，MOSS B.Analysis of a large cluster of nonessential genes deleted from a vaccinia virus terminal transposition mutant［J］.Virology，1988，167(2)：524-537.
［50］SHCHELKUNOV S N，SAFRONOV P F，TOTMENIN A V，et al.The genomic sequence analysis of the left and right species-specific terminal region of a cowpox virus strain reveals unique sequences and a cluster of intact ORFs for immunomodulatory and host range proteins［J］.Virology，1998，243(2)：432-460.
［51］MAVIAN C，LÓPEZ-BUENO A，BRYANT N A，et al.The genome sequence of ectromelia virus Naval and Cornell isolates from outbreaks in North America［J］.Virology，2014，462-463：218-226.
［52］SHCHELKUNOV S N，TOTMENIN A V，SAFRONOV P F，et al.Analysis of the monkeypox virus genome［J］.Virology，2002，297(2)：172-194.
［53］WANG Q，BURLES K，COUTURIER B，et al.Ectromelia virus encodes a BTB/kelch protein，EVM150，that inhibits NF-κB signaling［J］.J Virol，2014，88(9)：4853-4865.
［54］BEARD P M，FROGGATT G C，SMITH G L.Vaccinia virus kelch protein A55 is a 64 kDa intracellular factor that affects virus-induced cytopathic effect and the outcome of infection in a murine intradermal model［J］.J Gen Virol，2006，87(Pt6)：1521-1529.
［55］FROGGATT G C，SMITH G L，BEARD P M.Vaccinia virus gene F3L encodes an intracellular protein that affects the innate immune response［J］.J Gen Virol，2007，88(Pt7)：1917-1921.
［56］PIRES DE MIRANDA M，READING P C，TSCHARKE D C，et al.The vaccinia virus kelch-like protein C2L affects calcium-independent adhesion to the extracellular matrix and inflammation in a murine intradermal model［J］.J Gen Virol，2003，84(Pt9)：2459-2471.
［57］BALINSKY C A，DELHON G，AFONSO C L，et al.Sheeppox virus kelch-like gene SPPV-019 affects virus virulence［J］.J Virol，2007，81(20)：11392-11401.
［58］ZHANG L，VILLA N Y，RAHMAN M M，et al.Analysis of vaccinia virus-host protein-protein interactions：Validations of yeast two-hybrid screenings［J］.J Proteome Res，2009，8(9)：4311-4318.

[1]	陈孝军, 马俊, 陈熊男, 梁祎凡, 高琦, 黄钊, 许润达, 郑佳琛, 郑泽中, 张桂红, 王衡, 龚浪. 1株非洲猪瘟病毒p30蛋白单克隆抗体识别的B细胞抗原表位的鉴定[J]. 畜牧兽医学报, 2022, 53(7): 2239-2251.
[2]	于学祥, 陈晓雨, 李栋凡, 孙琪, 库旭钢, 范盛先, 杨汉春, 何启盖. 非洲猪瘟病毒无标签p30-ELISA抗体检测方法的建立及应用[J]. 畜牧兽医学报, 2022, 53(5): 1517-1526.