禽流感病毒PA-N182与鸡COPD蛋白互作的鉴定

doi:10.11843/j.issn.0366-6964.2015.10.025

畜牧兽医学报 ›› 2015, Vol. 46 ›› Issue (10): 1899-1904.doi: 10.11843/j.issn.0366-6964.2015.10.025

• 研究简报 • 上一篇

禽流感病毒PA-N182与鸡COPD蛋白互作的鉴定

王巧，李庆贺，刘冉冉，郑麦青，文杰，赵桂苹^*

（中国农业科学院北京畜牧兽医研究所，北京 100193）

收稿日期:2015-01-21 出版日期:2015-10-23 发布日期:2015-10-23
通讯作者: 赵桂苹，研究员，E-mail：zhaoguiping@caas.cn
作者简介:王巧(1991-)，女，山东日照人，硕士生，主要从事家禽抗病育种研究，E-mail：17746516692@163.com
基金资助:
中国农业科学院科技创新工程（ASTIP-IAS04）；国家科技支撑项目（2011BAD28B03）；国家肉鸡产业技术体系（CARS-42）

Identification of the Interaction of Avian Influenza Virus PA-N182 and COPD Protein of Chicken

WANG Qiao，LI Qing-he，LIU Ran-ran，ZHENG Mai-qing，WEN Jie，ZHAO Gui-ping^*

(Institute of Animal Science，Chinese Academy of Agricultural Sciences，Beijing 100193，China)

Received:2015-01-21 Online:2015-10-23 Published:2015-10-23

摘要/Abstract

摘要：

宿主蛋白质广泛参与流感病毒基因组在宿主体内的转录、复制和翻译。病毒蛋白质和宿主蛋白质的互作对于病毒的感染有重要影响。PA-N182是新发现的PA基因的转录形式，关于其宿主互作蛋白质的研究目前尚少。COPD（coatomer protein complex，subunit delta）是负责细胞内新合成蛋白质从内质网向高尔基体转运的基因，COPD基因敲除后细胞内流感病毒滴度降低，表明COPD基因可能参与流感病毒的复制。COPD基因影响流感病毒感染的机制仍未见报道。本研究通过构建PA-N182和COPD过表达载体并共转染细胞，利用免疫共沉淀、Western blot的方法，证实在鸡细胞内H5N1禽流感病毒PA-N182蛋白和COPD蛋白有相互作用，表明COPD对流感病毒感染的影响可能与其和PA-N182蛋白的互作并影响PA-N182蛋白从宿主细胞内质网向高尔基体的转运有着密切的联系。

Abstract:

Host fators are widely involved in transcription，duplication and translation of influenza virus genome，and host factors are reruired for the infection of influenza virus.PA-N182 is a recently identified trunctated form of influenza PA gene，and little is known about its host interaction factors.COPD(coatomer protein complex，subunit delta)，with the function of translocation of newly translated peptides from endoplasmic reticulum to Golgi apparatus，has an essential role in duplication of influenza virus and its knock down results in decrease of virus titer.We co-transfected PA-N182 and COPD expression vectors into chicken DF1 cells and found that PA-N182 interacted with COPD by the method of immunoprecipitation and Western blot.Our results reveal that COPD might affect the infection of influenza virus through ineracting with PA-N182 and translating it from endoplasmic reticulum to Golgi apparatus.

中图分类号:

王巧，李庆贺，刘冉冉，郑麦青，文杰，赵桂苹. 禽流感病毒PA-N182与鸡COPD蛋白互作的鉴定[J]. 畜牧兽医学报, 2015, 46(10): 1899-1904.

WANG Qiao，LI Qing-he，LIU Ran-ran，ZHENG Mai-qing，WEN Jie，ZHAO Gui-ping. Identification of the Interaction of Avian Influenza Virus PA-N182 and COPD Protein of Chicken[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(10): 1899-1904.

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参考文献

［1］SCHRAUWEN E J，DE GRAAF M，HERFST S，et al.Determinants of virulence of influenza A virus［J］.Eur J Clin Microbiol Infect Dis，2014，33(4)：479-490.
［2］JAGGER B W，WISE H M，KASH J C，et al.An overlapping protein-coding region in influenza A virus segment 3 modulates the host response［J］.Science，2012，337(6091)：199-204.
［3］SHI M，JAGGER B W，WISE H M，et al.Evolutionary conservation of the PA-X open reading frame in segment 3 of influenza A virus［J］.J Virol，2012，86(22)：12411-12413.
［4］SONG J，FENG H，XU J，et al.The PA protein directly contributes to the virulence of H5N1 avian influenza viruses in domestic ducks［J］.J Virol，2011，85(5)：2180-2188.
［5］YUAN P，BARTLAM M，LOU Z，et al.Crystal structure of an avian influenza polymerase PA（N） reveals an endonuclease active site［J］. Nature，2009，458(7240)：909-913.
［6］MURAMOTO Y，NODA T，KAWAKAMI E，et al.Identification of novel influenza A virus proteins translated from PA mRNA［J］.J Virol，2013，87(5)：2455-2462.
［7］YORK A，HUTCHINSON E C，FODOR E.Interactome analysis of the influenza A virus transcription/replication machinery identifies protein phosphatase 6 as a cellular factor required for efficient virus replication［J］.J Virol，2014，88(22)：13284-13299.
［8］DI PIETRO A，KAJASTE-RUDNITSKI A，OTEIZA A，et al.TRIM22 inhibits influenza A virus infection by targeting the viral nucleoprotein for degradation［J］. J Virol，2013，87(8)：4523-4533.
［9］XU X，KEDLAYA R，HIGUCHI H，et al.Mutation in archain 1，a subunit of COPI coatomer complex，causes diluted coat color and Purkinje cell degeneration［J］.PLoS Genet，2010，6(5)：e1000956.
［10］KÖNIG R，STERTZ S，ZHOU Y，et al.Human host factors required for influenza virus replication［J］.Nature，2010，463(7282)：813-817.
［11］SHI Y，WU Y，ZHANG W，et al.Enabling the ‘host jump’：structural determinants of receptor-binding specificity in influenza A viruses［J］.Nat Rev Microbiol，2014，12(12)：822-831.
［12］PFEFFERLE S，SCHÖPF J，KÖGL M，et al.The SARS-coronavirus-host interactome：identification of cyclophilins as target for pan-coronavirus inhibitors［J］.PLoS Pathog，2011，7(10)：e1002331.
［13］GALAN J A，PARIS L L，ZHANG H J，et al.Proteomic studies of Syk-interacting proteins using a novel amine-specific isotope tag and GFP nanotrap［J］.J Am Soc Mass Spectrom，2011，22(2)：319-328.

禽流感病毒PA-N182与鸡COPD蛋白互作的鉴定

Identification of the Interaction of Avian Influenza Virus PA-N182 and COPD Protein of Chicken

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