畜牧兽医学报 ›› 2015, Vol. 46 ›› Issue (6): 911-923.doi: 10.11843/j.issn.0366-6964.2015.06.006

• 遗传繁育 • 上一篇    下一篇

晋南牛与部分地方黄牛之间遗传多样性分析

王曦1,张元庆1*,贺东昌1,张喜忠1,李博1,王栋才1,靳光1,李福星2,杨效民1,徐芳1   

  1. (1.山西省农业科学院畜牧兽医研究所,太原 030032; 2.山西省运城市黄牛场,运城 044500)
  • 收稿日期:2014-07-28 出版日期:2015-06-23 发布日期:2015-06-23
  • 通讯作者: 张元庆,副研究员,E-mail:yuanqing_zhang@163.com
  • 作者简介:王曦(1975-),男,山西岚县人,副研究员,博士,主要从事牛遗传育种与功能基因组研究,E-mail:wxphilip@aliyun.com
  • 基金资助:

    “十二五”国家高技术研究发展计划(863计划)子课题(2011AA100307-05);山西省科技攻关项目(20130311024-1;20120311021-1;20130311024-2;20140311018-1);山西省农业科学院育种工程项目(11yzgc017);山西省农业科学院博士基金(YBSJJ1402)

Analyses of Genetic Diversity among Jinnan Cattle and Three other Chinese Indigenous Cattle Breeds

WANG Xi1,ZHANG Yuan-qing1* ,HE Dong-chang1,ZHANG Xi-zhong1,LI Bo1,WANG Dong-cai1,JIN Guang1,LI Fu-xing2,YANG Xiao-min1,XU Fang1   

  1. (1.Institute of Animal Science and Veterinary Medicine,Shanxi Academy of Agricultural Science,Taiyuan 030032 China;2.Yuncheng Yellow Cattle Farm,Yuncheng 044500,China)
  • Received:2014-07-28 Online:2015-06-23 Published:2015-06-23

摘要:

 旨在利用微卫星技术检测晋南牛、郏县红牛、鲁西牛和秦川牛四大地方黄牛的牛群遗传多样样及遗传结构。采用16个微卫星DNA标记对4个牛群进行检测分析。16个微卫星DNA标记中,除HEL9位点在所有牛群中呈单态外,其他15个位点的有效等位基因数为2~8个,平均有效等位基因数为3.067 6个。BM1818为低度多态(PIC=0.083 0,PIC<0.25),其余位点均为高度多态(PIC>0.5),其中HUAT24多态性最大(PIC=0.727 5)。4个牛群共检测到80个等位基因,其中晋南牛为63个,郏县红牛为65个,鲁西牛65个,秦川牛68个。在IDVGA46位点,仅有晋南牛有B等位基因,而在TGLA44位点,仅有晋南牛没有B等位基因。4个牛群的平均表观杂合度为0.385 2,平均期望杂合度为0.640 3,平均杂合度为0.578 0。晋南牛在NJ树上单独聚为一支,与鲁西黄牛、郏县红牛及秦川牛的遗传距离分别为0.809 3、0.759 8、0.807 1。结果表明,晋南牛遗传资源独特,群体遗传多样性丰富,是一个生长进化上较为封闭的群体。

Abstract:

The genetic diversity and genetic structure of Jinnan cattle,Jiaxian Red cattle,Luxi cattle and Qinchuan cattle were detected by microsatellite technology.Allele frequencies and distribution of 16 microsatellite markers were detected in 4 cattle populations.Number of effective alleles of the other 15 loci was from 2 to 8 except HEL9 as a monomorphic loci in all individuals,and the average number of effective alleles was 3.067 6.BM1818 was low genetic polymorphisms (PIC=0.083 0,PIC<0.25),and the others were high genetic polymorphisms (PIC>0.5),with the highest PIC value at HUAT24 locus (PIC=0.727 5).80 alleles were identified in 4 populations (63 alleles in Jinnan cattle,65 in Jiaxian Red cattle,65 in Luxi cattle and 68 in Qinchuan cattle,respectively).The allele B at IDVGA46 was detected only in Jinnan cattle,and the allele B at TGLA44 was only lack in Jinnan cattle.Mean observed heterozygosity,mean expected heterozygosity and mean heterozygosity were 0.385 2,0.640 3 and 0.578 0,respectively in 4 populations.Jinnan cattle was independently clustered in the NJ tree and the genetic distance (DA) with Luxi cattle,Jiaxian Red cattle and Qinchuan cattle were 0.809 3,0.759 8 and 0.807 1.There were special genetic characterics and sufficient genetic diversity in Jinnan cattle,and it was a relatively closed population in its evolutionary process.

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