晋南牛与部分地方黄牛之间遗传多样性分析

doi:10.11843/j.issn.0366-6964.2015.06.006

畜牧兽医学报 ›› 2015, Vol. 46 ›› Issue (6): 911-923.doi: 10.11843/j.issn.0366-6964.2015.06.006

晋南牛与部分地方黄牛之间遗传多样性分析

王曦¹，张元庆^1*，贺东昌¹，张喜忠¹，李博¹，王栋才¹，靳光¹，李福星²，杨效民¹，徐芳¹

(1.山西省农业科学院畜牧兽医研究所，太原 030032； 2.山西省运城市黄牛场，运城 044500)

收稿日期:2014-07-28 出版日期:2015-06-23 发布日期:2015-06-23
通讯作者: 张元庆，副研究员，E-mail：yuanqing_zhang@163.com
作者简介:王曦（1975-），男，山西岚县人，副研究员，博士，主要从事牛遗传育种与功能基因组研究，E-mail：wxphilip@aliyun.com
基金资助:
“十二五”国家高技术研究发展计划（863计划）子课题（2011AA100307-05）；山西省科技攻关项目（20130311024-1；20120311021-1；20130311024-2；20140311018-1）；山西省农业科学院育种工程项目（11yzgc017）；山西省农业科学院博士基金（YBSJJ1402）

Analyses of Genetic Diversity among Jinnan Cattle and Three other Chinese Indigenous Cattle Breeds

WANG Xi¹，ZHANG Yuan-qing^1* ，HE Dong-chang¹，ZHANG Xi-zhong¹，LI Bo¹，WANG Dong-cai¹，JIN Guang¹，LI Fu-xing²，YANG Xiao-min¹，XU Fang¹

（1.Institute of Animal Science and Veterinary Medicine，Shanxi Academy of Agricultural Science，Taiyuan 030032 China；2.Yuncheng Yellow Cattle Farm，Yuncheng 044500，China）

Received:2014-07-28 Online:2015-06-23 Published:2015-06-23

摘要/Abstract

摘要：

旨在利用微卫星技术检测晋南牛、郏县红牛、鲁西牛和秦川牛四大地方黄牛的牛群遗传多样样及遗传结构。采用16个微卫星DNA标记对4个牛群进行检测分析。16个微卫星DNA标记中，除HEL9位点在所有牛群中呈单态外，其他15个位点的有效等位基因数为2～8个，平均有效等位基因数为3.067 6个。BM1818为低度多态（PIC=0.083 0，PIC<0.25），其余位点均为高度多态（PIC>0.5），其中HUAT24多态性最大（PIC=0.727 5）。4个牛群共检测到80个等位基因，其中晋南牛为63个，郏县红牛为65个，鲁西牛65个，秦川牛68个。在IDVGA46位点，仅有晋南牛有B等位基因，而在TGLA44位点，仅有晋南牛没有B等位基因。4个牛群的平均表观杂合度为0.385 2，平均期望杂合度为0.640 3，平均杂合度为0.578 0。晋南牛在NJ树上单独聚为一支，与鲁西黄牛、郏县红牛及秦川牛的遗传距离分别为0.809 3、0.759 8、0.807 1。结果表明，晋南牛遗传资源独特，群体遗传多样性丰富，是一个生长进化上较为封闭的群体。

Abstract:

The genetic diversity and genetic structure of Jinnan cattle，Jiaxian Red cattle，Luxi cattle and Qinchuan cattle were detected by microsatellite technology.Allele frequencies and distribution of 16 microsatellite markers were detected in 4 cattle populations.Number of effective alleles of the other 15 loci was from 2 to 8 except HEL9 as a monomorphic loci in all individuals，and the average number of effective alleles was 3.067 6.BM1818 was low genetic polymorphisms (PIC=0.083 0，PIC<0.25)，and the others were high genetic polymorphisms (PIC＞0.5)，with the highest PIC value at HUAT24 locus (PIC=0.727 5).80 alleles were identified in 4 populations (63 alleles in Jinnan cattle，65 in Jiaxian Red cattle，65 in Luxi cattle and 68 in Qinchuan cattle，respectively).The allele B at IDVGA46 was detected only in Jinnan cattle，and the allele B at TGLA44 was only lack in Jinnan cattle.Mean observed heterozygosity，mean expected heterozygosity and mean heterozygosity were 0.385 2，0.640 3 and 0.578 0，respectively in 4 populations.Jinnan cattle was independently clustered in the NJ tree and the genetic distance (D_A) with Luxi cattle，Jiaxian Red cattle and Qinchuan cattle were 0.809 3，0.759 8 and 0.807 1.There were special genetic characterics and sufficient genetic diversity in Jinnan cattle，and it was a relatively closed population in its evolutionary process.

中图分类号:

S813.1
S823

王曦，张元庆，贺东昌，张喜忠，李博，王栋才，靳光，李福星，杨效民，徐芳. 晋南牛与部分地方黄牛之间遗传多样性分析[J]. 畜牧兽医学报, 2015, 46(6): 911-923.

WANG Xi，ZHANG Yuan-qing，HE Dong-chang，ZHANG Xi-zhong，LI Bo，WANG Dong-cai，JIN Guang，LI Fu-xing，YANG Xiao-min，XU Fang. Analyses of Genetic Diversity among Jinnan Cattle and Three other Chinese Indigenous Cattle Breeds[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(6): 911-923.

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晋南牛与部分地方黄牛之间遗传多样性分析

Analyses of Genetic Diversity among Jinnan Cattle and Three other Chinese Indigenous Cattle Breeds

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