NOBOX基因在麦洼牦牛卵母细胞、早期胚胎及胎儿卵巢的表达

doi:10.11843/j.issn.0366-6964.2014.11.002

畜牧兽医学报 ›› 2014, Vol. 45 ›› Issue (11): 1746-1751.doi: 10.11843/j.issn.0366-6964.2014.11.002

NOBOX基因在麦洼牦牛卵母细胞、早期胚胎及胎儿卵巢的表达

符梅^1#，陈亚冰^1#，李键^2*，熊显荣¹,兰道亮²

（1.西南民族大学生命科学与技术学院，动物遗传育种重点实验室，成都 610041；2.西南民族大学青藏高原研究院，成都 610041）

收稿日期:2014-05-16 出版日期:2014-11-23 发布日期:2014-11-23
通讯作者: 李键，教授，博士，主要从事动物生殖调控研究，E-mail：lijian@swun.cn
作者简介:符梅(1988-)，女，海南儋州人，硕士生，主要从事细胞与胚胎工程研究，E-mail:meizi880716@126.com；陈亚冰（1989-），男，河南许昌人，硕士生，主要从事动物生理病理学研究，E-mail:cybxc923410296@126.com；符梅和陈亚冰并列为第一作者
基金资助:
国家科技支撑计划项目（2012BAD13B06）；西南民族大学中央高校项目（82001350）专项

NOBOX Gene Expression in Maiwa Yak Oocytes，Early Embryogenesis and Fetal Ovaries

FU Mei^1#，CHEN Ya-bing^1#，LI Jian^2*，XIONG Xian-rong¹，LAN Dao-liang²

(1.College of Life Science and Technology，Southwest University for Nationalities，Chengdu 610041,China;2.College of Tibetan Plateau Research，Southwest University for Nationalities，Chengdu 610041,China)

Received:2014-05-16 Online:2014-11-23 Published:2014-11-23

摘要/Abstract

摘要：

旨在研究牦牛胚胎发育过程中NOBOX基因表达与卵母细胞、胚胎发育及胎儿卵巢的关系。利用RT-PCR技术检测NOBOX基因在颗粒细胞、牦牛胎儿卵巢及牦牛各组织中的表达；并采用荧光定量PCR技术检测NOBOX基因在卵母细胞体外受精胚胎发育过程中（GV期、MII期、2-细胞、4-细胞、8-细胞、16-细胞、桑椹胚和囊胚）的相对表达变化。结果表明，NOBOX基因在牦牛各组织中均有表达，其中肾表达量最高。在GV期卵泡中，NOBOX基因仅在卵母细胞中有表达，颗粒细胞中未检测到表达。早期胚胎发育过程中，随着胚胎发育，其表达量逐渐降低，在GV期时表达量最高（P<0.05），随后逐渐降低，在16-细胞表达量最低并趋于稳定。胎牛发育成长过程中，NOBOX基因在胎儿卵巢中表达量随着胎儿年龄的增长表达呈上升趋势。综上表明，NOBOX基因在牦牛早期胚胎发育过程中mRNA表达量存在差异性，可能与早期胚胎的正常发育和不同的生理活动有关。

Abstract:

The objective of this study was to investigate the correlation between yak NOBOX gene expression and oocytes，embryogenesis and fetal ovaries.RT-PCR was used to detect the expression pattern of NOBOX gene in yak fetal ovaries，granulose cells and various tissues；Real-time PCR was chosen to analyze the dynamic changes of NOBOX gene expression during oocyte maturation and early embryonic development (GV，MII，2-cell，4-cell，8-cell，16-cell，MO and BL).The results showed that NOBOX gene was expressed in various tissues of yak，and highest in kidney.At GV-stage ovarian follicle，the expression of NOBOX gene was detected only in oocytes not in granule cells.Moreover，NOBOX gene was expressed regularly during early embryonic development，which has a highest level at GV-stage (P<0.05)，decreasing with the embryonic development，and attained a steady level at 16-cell stage.However，it’s noteworthy that the expression of NOBOX gene in ovary increased with yak fetal growth and development.The different expression pattern of NOBOX gene in different stages of early embryonic development may be associated with embryonic development and various physiological activities.

中图分类号:

Q785

符梅，陈亚冰，李键，熊显荣,兰道亮. NOBOX基因在麦洼牦牛卵母细胞、早期胚胎及胎儿卵巢的表达[J]. 畜牧兽医学报, 2014, 45(11): 1746-1751.

FU Mei，CHEN Ya-bing，LI Jian，XIONG Xian-rong，LAN Dao-liang. NOBOX Gene Expression in Maiwa Yak Oocytes，Early Embryogenesis and Fetal Ovaries[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(11): 1746-1751.

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NOBOX基因在麦洼牦牛卵母细胞、早期胚胎及胎儿卵巢的表达

NOBOX Gene Expression in Maiwa Yak Oocytes，Early Embryogenesis and Fetal Ovaries

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