玉米赤霉烯酮对小鼠睾丸间质细胞内StAR蛋白及类固醇合成关键酶表达的影响

doi:10.11843/j.issn.0366-6964.2014.07.009

畜牧兽医学报 ›› 2014, Vol. 45 ›› Issue (7): 1091-1096.doi: 10.11843/j.issn.0366-6964.2014.07.009

玉米赤霉烯酮对小鼠睾丸间质细胞内StAR蛋白及类固醇合成关键酶表达的影响

郑王龙^1,2，刘青^1,3，王亚军¹，黄沁怡¹，徐辉¹，王宜安¹，顾建红¹，袁燕¹，刘学忠¹，刘宗平^1,2，卞建春^1,2*

（1.扬州大学兽医学院，扬州 225009； 2.江苏省动物重要疫病与人兽共患病防控协同创新中心，扬州 225009；3.焦作出入境检验检疫局，焦作 454001）

收稿日期:2014-01-22 出版日期:2014-07-23 发布日期:2014-07-23
通讯作者: 卞建春，教授，E-mail：jcbian@yzu.edu.cn
作者简介:郑王龙（1988-），男，江西上饶人，硕士生，主要从事动物营养代谢病与中毒病研究，E-mail：keeporkeep@163.com
基金资助:
江苏高校优势学科建设工程资助项目(PAPD)；江苏省普通高校自然科学基金项目(08KJD230002);江苏省研究生培养创新工程项目(CXLX13_921)

Effects of Zearalenone on the Expression of Steroidogenic Acute Regulatory Protein and Key Enzymes in Primary Leydig Cells

ZHENG Wang-long^1,2，LIU Qing^1，3，WANG Ya-jun¹，HUANG Qin-yi¹，XU Hui¹，WANG Yi-an¹，GU Jian-hong¹，YUAN Yan¹，LIU Xue-zhong¹，LIU Zong-ping^1,2，BIAN Jian-chun^1,2*

（1.College of Veterinary Medicine,Yangzhou University,Yangzhou 225009,China; 2.Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses，Yangzhou 225009,China; 3.Jiaozuo Entry-Exit Inspection and Quarantine Bureau,Jiaozuo 454001,China)

Received:2014-01-22 Online:2014-07-23 Published:2014-07-23

摘要/Abstract

摘要：

为了探讨玉米赤霉烯酮（Zearalenone，ZEA）抑制睾丸间质细胞分泌雄激素的毒性机理，本研究以体外培养的原代小鼠睾丸间质细胞为材料，分别以0 (对照组)、1、5、10、20 μg•mL^-1的ZEA进行染毒，染毒12 h后，利用Western-blot和QRT-PCR技术，检测了细胞内类固醇合成快速调节蛋白（StAR）和类固醇合成关键酶(P450scc、3β-HSD、P450c17a1和17β-HSD)在转录和蛋白水平的表达。结果显示，与对照组相比，随着ZEA染毒浓度的升高，StAR转录水平及蛋白水平的相对表达量都呈上升趋势（P<0.05或P<0.01)；而类固醇关键酶P450scc、3β-HSD、P450c17a1、17β-HSD的转录水平及蛋白水平的相对表达量都呈下降趋势（P<0.05或P<0.01)。结果表明：ZEA抑制睾丸间质细胞分泌睾酮的一个重要原因是其抑制了睾酮合成通路中类固醇合成关键酶的分泌。

Abstract:

In order to investigate the toxic mechanim of the inhibition of androgen secretion induced by ZEA (Zearalenone）in leydig cells，the primary leydig cells，isolated in vitro，were exposed to 0 (control group)，1，5，10，20 μg•mL^-1 ZEA for 12 h，respectively. Western-blot and QRT-PCR were used to measure the expressions of Steroidogenic acute regulatory protein（StAR）,P450scc,3β-HSD,P450c17a1 and 17β-HSD mRNA and the related proteins. The results showed that the transcription of the mRNA of StAR and the expression of the related proteins were increased dramatically (P<0.05，P<0.01) depended on the concentration of ZEA. In contrast，the transcription of the mRNA of P450scc,3β-HSD,P450c17a1 and 17β-HSD and the expression of the related proteins were decreased significantly (P<0.05,P<0.01).In conclusion，the key enzymes of steroid secretion in the pathway of testosterone synthesis played an important role in the inhibition of androgen secretion induced by ZEA (Zearalenone） in leydig cells.

中图分类号:

S865.1⁺3.3

郑王龙，刘青，王亚军，黄沁怡，徐辉，王宜安，顾建红，袁燕，刘学忠，刘宗平，卞建春. 玉米赤霉烯酮对小鼠睾丸间质细胞内StAR蛋白及类固醇合成关键酶表达的影响[J]. 畜牧兽医学报, 2014, 45(7): 1091-1096.

ZHENG Wang-long，LIU Qing，WANG Ya-jun，HUANG Qin-yi，XU Hui，WANG Yi-an，GU Jian-hong，YUAN Yan，LIU Xue-zhong，LIU Zong-ping，BIAN Jian-chun. Effects of Zearalenone on the Expression of Steroidogenic Acute Regulatory Protein and Key Enzymes in Primary Leydig Cells[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(7): 1091-1096.

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玉米赤霉烯酮对小鼠睾丸间质细胞内StAR蛋白及类固醇合成关键酶表达的影响

Effects of Zearalenone on the Expression of Steroidogenic Acute Regulatory Protein and Key Enzymes in Primary Leydig Cells

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