清道夫受体在树突状细胞提呈口蹄疫病毒VP1-VP4抗原中的作用

doi:10.11843/j.issn.0366-6964.2014.01.018

畜牧兽医学报 ›› 2014, Vol. 45 ›› Issue (1): 129-135.doi: 10.11843/j.issn.0366-6964.2014.01.018

清道夫受体在树突状细胞提呈口蹄疫病毒VP1-VP4抗原中的作用

高云欢，李娜，董昌海，唐然肖，李丽敏，王家鑫^*

(河北农业大学动物医学院免疫学实验室,保定 071000)

收稿日期:2013-07-22 出版日期:2014-01-23 发布日期:2014-01-23
通讯作者: 王家鑫，教授，E-mail: mastwang@163.com
作者简介:高云欢（1988-），男，河北无极人，硕士生，主要从事抗原提呈机制的研究；李娜（1987-），女，河北保定人，硕士生，主要从事抗原提呈机制的研究。二人并列第一作者
基金资助:
国家自然科学基金资助项目（30972168）

The Role of Scavenger Receptors on Dendritic Cells in Presenting VP1-VP4 Antigen of Foot-and-Mouth Disease Virus to T Cells

GAO Yun-huan, LI Na, DONG Chang-hai, TANG Ran-xiao, LI Li-min, WANG Jia-xin^*

(College of Veterinary Medicine, Agricultural University of Hebei, Baoding 071000, China)

Received:2013-07-22 Online:2014-01-23 Published:2014-01-23

摘要/Abstract

摘要：

为研究清道夫受体在树突状细胞提呈口蹄疫病毒VP1-VP4融合蛋白中的作用，构建了pET-32a-VP1-VP4原核表达系统，以此获得VP1-VP4融合蛋白。制备骨髓源树突状细胞(BMDCs)和淋巴结T细胞，首先用清道夫受体抑制剂poly（I）处理BMDCs，然后再将VP1-VP4融合蛋白负载经poly（I）处理后的BMDCs，并与淋巴结T细胞共培养，收集不同时间点的共培养上清液，用ELISA检测其中IFN-γ含量。结果显示，经poly（I）处理的试验组在每个时间点产生的IFN-γ含量均明显高于对照组，且差异极显著（P＜0.01）。表明清道夫受体可识别口蹄疫病毒VP1-VP4融合蛋白，并在BMDCs提呈VP1-VP4抗原的过程中发挥负调节作用。

Abstract:

The aim of this study was to investigate the role of the scavenger receptors on dendritic cells in presentation of recombinant VP1-VP4 of foot-and-mouth disease virus (FMDV) to T cells in vitro. The prokaryotic expression vector of pET-32a-VP1-VP4 was constructed and the VP1-VP4 protein was expressed and purified. Bone marrow-derived dendritic cells (BMDCs) pretreated with scavenger receptor inhibitors were pulsed with recombinant VP1-VP4 antigen and then cocultured with lymph node T cells. The culture supernatants were harvested at different time points for
determining IFN-γ contents with ELISA. The results showed that the IFN-γ levels of experimental groups were significantly higher than that of control group at different time points (P＜0.01). Our data indicate that scavenger receptors on BMDCs are able to recognize recombinant VP1-VP4 protein and play a negative regulatory role in antigen presentation of BMDCs to initiate lymph node T cell responses.

中图分类号:

">S852.659.6

高云欢，李娜，董昌海，唐然肖，李丽敏，王家鑫. 清道夫受体在树突状细胞提呈口蹄疫病毒VP1-VP4抗原中的作用[J]. 畜牧兽医学报, 2014, 45(1): 129-135.

GAO Yun-huan, LI Na, DONG Chang-hai, TANG Ran-xiao, LI Li-min, WANG Jia-xin. The Role of Scavenger Receptors on Dendritic Cells in Presenting VP1-VP4 Antigen of Foot-and-Mouth Disease Virus to T Cells[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(1): 129-135.

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清道夫受体在树突状细胞提呈口蹄疫病毒VP1-VP4抗原中的作用

The Role of Scavenger Receptors on Dendritic Cells in Presenting VP1-VP4 Antigen of Foot-and-Mouth Disease Virus to T Cells

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