妊娠大鼠子宫和胎盘MHC类分子的表达及TGF-β1对其表达的影响

doi:10.11843/j.issn.0366-6964.2013.05.019

畜牧兽医学报 ›› 2013, Vol. 44 ›› Issue (5): 802-810.doi: 10.11843/j.issn.0366-6964.2013.05.019

妊娠大鼠子宫和胎盘MHC类分子的表达及TGF-β1对其表达的影响

李明^*，张俊华，孙兆军，陈卫民

（宁夏大学新技术应用研究开发中心，银川 750021）

收稿日期:2012-09-06 出版日期:2013-05-23 发布日期:2013-05-23
通讯作者: 李明，E-mail:lm_y096@126.com
作者简介:李明（1980-），女，汉族，宁夏中卫人，助理研究员，博士生,主要从事兽医产科学研究，Tel：0951-2062518，E-mail：lm_y096@126.com
基金资助:
宁夏自然科学基金项目(NZ12125)

The Expression of MHC in Uterus and Placenta of Pregnant Rat and Its Regulation by TGF-β1

LI Ming^*, ZHANG Jun-hua, SUN Zhao-jun, CHEN Wei-min

(Development Center of New Technique Application and Research, Ningxia University,Yinchuan 750021, China)

Received:2012-09-06 Online:2013-05-23 Published:2013-05-23

摘要/Abstract

摘要：

本研究旨在对大鼠妊娠过程中子宫和胎盘主要组织相容性抗原（MHC）的动态表达和表达定位，及体内注射超生理剂量的转化生长因子-β1（TGF-β1）后MHC分子的表达进行检测，探讨妊娠子宫和胎盘中典型性MHC-Ⅰ类分子（RT1-A）和非典型性MHC-Ⅱ类分子（RT1-DM）的表达规律、定位及与TGF-β1的关系。笔者采用蛋白质印迹和免疫组化方法对妊娠各时期大鼠子宫和胎盘中相关指标进行检测。结果表明，正常妊娠各个时期大鼠子宫和胎盘中均能检测到MHC类分子的表达。子宫中RT1-A蛋白的表达在整个妊娠期呈增长趋势（D4~D19）。妊娠早期至妊娠中期RT1-DM蛋白在子宫中的表达量逐渐增加，妊娠晚期表达量下降。胎盘中RT1-A、RT1-DM蛋白的表达量自妊娠早期至妊娠中期下降，妊娠晚期逐渐上升。注射超生理剂量的TGF-β1后，植入前期子宫中RT1-A略有升高，在植入期（D6）和植入后期（D9）下降，D19显著下降（P<0.01）。RT1-DM表达量在植入前期上升、植入期下降、植入后期略有上升，妊娠晚期显著下降（P<0.01）。胎盘中妊娠早期（D9）和晚期（D19）RT1-A和RT1-DM的表达显著下降（P<0.01），妊娠中期这两种蛋白表达量显著上升（P<0.05，P<0.01）。免疫组化结果表明这两类分子的定位不受外源性TGF-β1的影响：妊娠早期RT1-DM主要定位于子宫腔上皮、腺上皮、血管壁和子宫肌层。RT1-A主要定位于腔上皮、腺上皮、基蜕膜及血管壁。妊娠中晚期RT1-A和RT1-DM主要定位于迷宫层、海绵滋养层、腺上皮和子宫血管壁。综上所述，大鼠妊娠过程中超生理剂量的TGF-β1对MHC抗原表达有调节作用，这种调节在妊娠各个时期有所不同。

Abstract:

The aim of this study was to investigate MHC expression and localization in uterus and placenta of pregnancy rat, and to study the possible mechanism of immunomodulation classical class Ⅰmajor histocompatibility complex (RT1-A) antigens and non-classical classⅡ major histocompaibility complex (RT1-DM) antigens influenced by exogenous TGF-β1 (Transforming Growth Factor-β1). All of these parameters were examined by western blot and immunohistochemistry. The results showed that the expression of RT1-A and RT1-DM protein were examined in uterus and placenta during every stage of gestation. The expression of RT1-A protein was increasing gradually at uterus during all stage of gestation (D4-D19). The expression of RT1-DM protein was increasing at uterus from early-stage to mid-stage of gestation and decreasing in late-stage. In placenta, the expression of RT1-A, RT1-DM were decreasing gradually during mid-stage to late-stage of gestation. TGF-β1 treatment increased expression of RT1-A and RT1-DM in pre-implantation stage and decreased them in implantation stage (D6-D9) and late at uterus. During late-stage gestation (D19) TGF-β1 treatment significantly inhibited the expression of RT1-A and RT1-DM protein at uterus (P<0.01). The expression of RT1-A and RT1-DM was up-regulated during mid gestation significantly (P<0.05, P<0.01). Immunohistochemistry showed that TGF-β1 did not affect the localization of the two molecules: in early pregnancy RT1-DM mainly localized on uterine luminal epithelium, glandular epithelium, blood vessels, myometrium. RT1-A mainly localized on decidual blood vessels luminal epithelium, glandular epithelium, dicidualbasalis. During mid and late pregnancy, RT1-A and RT1-DM mainly localized on labyrinthine zones, spongiotrophoblast, glandular epithelium, uterus blood vessels. These results indicated that the expression of MHC antigens and TGF-β1 in maternal-fetal interface which may be helpful for constructing an appropriate micro-immunoenvironment of fetal parturition during normal pregnancy.

中图分类号:

R714.1

李明，张俊华，孙兆军，陈卫民. 妊娠大鼠子宫和胎盘MHC类分子的表达及TGF-β1对其表达的影响[J]. 畜牧兽医学报, 2013, 44(5): 802-810.

LI Ming, ZHANG Jun-hua, SUN Zhao-jun, CHEN Wei-min. The Expression of MHC in Uterus and Placenta of Pregnant Rat and Its Regulation by TGF-β1[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(5): 802-810.

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妊娠大鼠子宫和胎盘MHC类分子的表达及TGF-β1对其表达的影响

The Expression of MHC in Uterus and Placenta of Pregnant Rat and Its Regulation by TGF-β1

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