ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2014, Vol. 45 ›› Issue (10): 1567-1583.doi: 10.11843/j.issn.0366-6964.2014.10.001

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Research Progress on CRISPR/Cas9 Mediated Genome Editing

LIU Zhi-guo*   

  1. (Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193,China)
  • Received:2014-04-17 Online:2014-10-23 Published:2014-10-23

Abstract:

Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein 9 (Cas9) constituted the CRISPR/Cas9 genome editing system,which provides a more efficient way for gene targeting than zinc finger endonuclease (ZFN) and transcription activator-like effector nuclease (TALEN).It is becoming a hot spot in the field of genome editing,and successfully applied to gene targeting of bacteria,human cells,zebra fish,mouse,pig,cynomolgus monkey and several plants.However,the high off-target rate and poor specificity of CRISPR/Cas9 technology hindered its application in medical treatment.Here,the research progress in the structure,classification,function,and mechanism of the CRISPR/Cas9 system is reviewed.

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