Methodology of QTL mapping for ordinal traits of disease resistance basing on the framework of a generalized linear model (GLM) was present in the paper. The location and effect parameters of putative QTL were estimated using Maximum Likelihood method. The efficiency and power were compared with the linear model (LM). The factors of influencing QTL detection efficiency (e.g. QTL effect and heritability) were simulated in our study too. Daughter design with multiple families was applied, and the number of segregating population is 500. The results showed that threshold model have a great advantage in location estimation and power of QTL mapping, and have nice efficiency and accuracy for ordinal traits. In addition, the accuracy of QTL mapping depended on the effect of putative quantitative trait loci and the value of heritability. With the increase of QTL effect and heritability, the accuracy of QTL mapping improved slightly.

A 374bp fragment spanning over exon 2 of major histocompatibility complex B-LBⅡ gene was amplified, cloned and sequenced in eight Chinese indigenous chicken breeds and one introduced breed. Twenty new B-LBⅡalleles were found in the chicken breeds sampled. The polymorphism of the exon 2 was investigated by PCR-RFLP technique at the six loci of AluⅠ, CaiⅠ, CfrⅠ, Hin1Ⅰ,HinfⅠand RsaⅠ. The observed homozygous genotypes at the six loci were utilized to tentatively identify the alleles of B-LBⅡ genes in the animals analyzed. It indicated that 65% of alleles were discernible by this method, and the alleles accounted for 68.75% of major type alleles sampled. Alignment of the exon 2 sequences of 7 haplotypes and the 20 alleles revealed 63 polymorphic sites, of which 48 sites were parsimony informative, and 15 sites were singleton variable. Among the variable sites, 13 of parsimony informative sites and 11 out of the singleton variable sites were unique to the Chinese indigenous breeds sampled. The sequences of the exon 2 shared 90.6%~99.5% homology to each other. The relative frequencies of nonsynonymous and synonymous substitutions of nucleotide at per site in the exon 2 were estimated, 14.64%±2.67% and 2.92%±0.94%, respectively; the former was highly exceeded the latter. The present study provided the basic data for investigation of the evolutionary mechanisms of MHC B-LBⅡ genes in chicken.

9 microsatellite loci were used to detect mean heterozygosity, polymorphism information content(PIC) and genetic relationships in 11 duck populations. The results showed that mean heterozygosity of 11 duck populations was 0.659 9, and mean PIC was 0.596 6. Among the 11 duck populations, mean heterozygosity of the Black Feather Muscovy（white hair）was the highest (0.694 8), and that of Jingjiang Partridge duck was the lowest(0.575 7). There were similar results in PIC. The 11 duck populations were divided into three group by NJ phylogenetic tree based on the genetic distance. The first group included White Feather Muscovy duck, Black Feather Muscovy duck, Black Feather Muscovy duck（white hair）;the second group included Shan Partridge duck, Jinding duck, Gaoyou duck; and the third group included Sumu White duck, Sumu Black duck, Longbai duck, Sumu Partridge duck, Jingjiang Partridge duck.

Among the genes that are involved with the development of growth and development of fat tissues, PPAR subfamily members are one cluster of the most important genes. The object of this study were to identify polymorphism of PPARα gene in a AA broiler line, develop PCR-SSCP methods to detect those DNA polymorphism and evaluate the relationship between those polymorphism PPARα genes and body fat traits. The single nucleotide polymorphism (SNP) were found in exon regions of the PPARα. The differential genotype detected by PCR-SSCP were defined as EE, EF and FF. The statistics analysis results showed that the PPARα polymorphism was significant related to the abdominal fat（AF）weight and abdominal fat percentage（AFP）(P＜0.05), and the chicken with the genotype of FF has a higher AF and AFP than that of EE; while there was no significant difference between the genotype of FF and EF. The chicken with the genotype of EF has a higher AF and AFP than that of EE, but did not attain the significant difference level （P＞0.05）. The result indicated that the PPARα play an important role in chicken fat tissue metabolism. The significantly correlation between PPARα and development of fat tissue suggested that this gene is a good marker for using in marker associated selection program.

The study was designed to carry out DNA polymorphism analysis of a total of 70 Alpine utra-fine wool sheep (Chinese Merino) using 3 pairs of microsatellite primers. The results showed that 3 microsatellite Loci of the Alpine ultrafine wool sheep had high diversity, with the average polymorphism information content of 0.739 1 and the number of effective alleles of 4.441 9. The result of the correlation analysis between genetic diversity of microsatellite DNA and economic traits showed that both 160bp alleles of MCM 218 and 288bp alleles of BMC 1009 were highly correlated with wool fitness (P<0.01), with a correlation coefficient of -0.956 1 and -0.849 1 respectively; 145bp alleles of BMS1248 were also highly correlated with yearling weight(P<0.05), with a correlation coefficient of 0.952 3. This indicates that there is a linkage between the genetic diversity of microsatellite DNA and economic traits of Alpine ultra-fine wool sheep. Therefore, this is a fundamental study for further looking for markers of both linkage and cloning, and for early selection of the important economic traits of Alpine ultrafine wool Chinese merino.

According to the amplification character of polymerase in polymerase chain reaction, the PCR circulating parameters were studied and the extension temperature gradient was eliminated during amplification. The two-temperature gradient PCR method was used not only in both single PCR and multiplex PCR amplification study, but also in the PCR amplification for bovine samples of genome, fibroblast, embryos respectively. At last, the stable, simple, fast method of two-temperature gradient PCR for sexing bovine pre-implantation embryos was obtained. At the same time, the amplification ability of two-temperature gradient multiplex PCR was detected, as a result, it indicated that less than 633bp chain could been synthesized successfully in the two-temperature gradient PCR condition.

The major object of this paper is to isolate and culture bovine embryonic germ (EG) cells derived from primordial germ cells (PGCs) as well as to identify the EG cells from various aspects. Some factors influencing the efficiency of the isolation and culture bovine EG cells have been discussed. When co-culture the EG cells with somatic cells, there will be some colony in primary culture, which maybe account for homologous somatic cells can sustain the EG survive and proliferation. When we use the tissue culture method, the colony of EG cells is very less and EG cells cannot proliferate effectively, as well as the passage culture. With 0.125% trypsinase + 0.02% EDTA disrupt gonad, comparing effect of different dispersal methods on the passage of the EG cells, the method of “digest + blowing disperse” was fine, which was simple and can save more time and labours and better to keep the activity of the EG cells. An Immunohistochemistry assay was carried out to characterize the colonies of bovine primory EG cells, and expression of stage-specific embryonic antigen SSEA-1, 3, 4 was detected, but the expression was feeble. The EG cells can differentiate into fibroblast-like cells, epithelia-like cells and embryoid body.

The goat 8~16C stage embryos obtained from in vivo and in vitro was studied using the way of single preimplantation embryo mRNA differential display. Some specific fragments expressed in vivo embryos was found and one of them was selected for further analysis. The result suggested that this fragment displayed high homology (91%) to human mRNA for ribosomal protein L31. The protein encoded by this gene is one element of ribosomal. The integrated structure of ribosomal is essential for the process of protein synthesis, and the ribosomal proteins have the important functions of regulating the gene expression as well.

Rumen ciliate species and composition were surveyed on Murrah Buffalo, and Xilin Buffalo, in Guangxi, China. The concentrations of volatile fatty acids of rumen fluid were detected. As a result of survey, 17 genera including 63 species with 25 formae were identified. Total number and average genus number of rumen ciliate in this survey were more than those in 8 ruminants which were studied long time ago. The composition of species was similar to that of buffalo rumen in Southeast Asia. A new species of the genus Entodinium was recognized from Xilin Buffalo, and then it was described as Entodinium biconcavum sp n. This new species was not detected before. 13 genera including 54 species with 20 formae, and 16 genera including 45 species with 11 formae were identified from Xilin Buffalo, Murrah Buffalo, respectively. The average ciliate density was estimated as 2.13×10⁵/mL in Xilin Buffalo, 3.43×10⁵/mL in Murrah Buffalo. The genus Entodinium was recognized with highest frequency of appearance in Xilin Buffalo and Murrah Buffalo rumen ciliate, but that of Xilin Buffalo was different from that of Murrah Buffalo. Other genera were not the same, which was related to buffalo kept in environment and feeding system. The total volatile fatty acids concentration of rumen fluid of Xilin Buffalo, and Murrah Buffalo were 75.60 mmol/L, and 58.74 mmol/L respectively. The concentration of acetate acid of rumen fluid was highest among VFAs, suggesting that rumen fermentation was typical forage fermentation.

Four Xuhuai white goats fitted with permanently ruminal cannulae,proximal duodenal and terminal ileal T-shape cannulaes were used in a 4×4 latin square design to determine the effects of four different ratios of neutral detergent fiber to N-free extract(NDF/NFC)(NDF/NFC were 2.75(A),1.71(B), 1.12(C) and 0.74(D) respectively) in diets on the absorbable amino acids(AAA) of the goats.The study used DAPA and PC respectively as internal marker of rumen bacteria and protozoa to measure the flux and composition of all the AA of bacteria and protozoa to the duodenum.The results showed that:（1）Of the AAA in the small intestine,the flow of bacterial AA in group B was 1.54g/d,which was much higher than group A(0.45 g/d),C(0.36 g/d)and D(0.39 g/d)(P<0.01).The proportion of bacterial AA in group B to the total AAA was 38.4%,which was higher than group D(24.84%)(P<0.05),and significantly higher than group A(13.25%) and C(14.81%)(P<0.01);（2）The flow of protozoal AA in group A was 1.22 g/d,significantly higher than in group B(0.63 g/d),C(0.57 g/d) and D(0.42 g/d)(P<0.01),furthermore, the proportion of that in group A to the total AAA was 36.74%,while group B,C and D was 15.71%,23.45% and 26.75% respectively;（3）Of the AAA from bypass protein,the flow of group A,B was 1.65 g/d,1.84 g/d, both of them were higher than C(1.51 g/d)(P<0.05),and significantly higher than D(0.76 g/d)(P<0.01).The highest proportion to the total AAA was observed in group C(61.37%),which was higher than A(49.13%),D(48.41%)(P<0.05),and significantly higher than B(45.88%)(P<0.01),i.e.the efficiency of dietary AA in the rumen transforming into microbial protein in group B was the greast;（4）Among the AA composition of microbial protein,the AA of protozoa was relatively stable,while the production and composition of Solid Associated Bacteria(SAB) was more stable than that of Liquid Associated Bacteria(LAB).The composition and quantity of the bacterial AAA in the small intestine were directly affected by the change of LAB.

This study investigated the effects of photoperiod and melatonin on nitrogen partitioning in Inner Mongolia White Cashmere goats in telogen in Inner Mongolia, north China. 18 castrated mature goats, 23~25 kg, were divided into three groups at random, each group including 6 goats were fed in pens in different rooms, which were treated with long daily photoperiod (LDPP：16L，8D), short daily photoperiod (SDPP：8L，16D) and natural daily photoperiod (NDPP), respectively. The 3 goats in each group were implanted melatonin (1.68 mg/kg) monthly from February 20 to June 20. Total deposited nitrogen (TN) was tested by general digestive and metabolism method. Body nitrogen deposited (BN) was measured by dilution technic of tritiated water at the beginning and the end of the experiment, cashmere and hair nitrogen deposited(C&HN) were calculated with C&HN= TN-BN. Results showed there was a significant difference between LDPP and SDPP in BN and C&HN. C&HN in SDPP was higher than that of LDPP evidently, the corresponding was 33.7%±0.64% vs 23.6%±0.46% (P<0.01), on the other hand, BN in SDPP was much lower than that of LDPP, the corresponding was 66.3%±0.64% vs 76.4%±0.46% (P<0.01). Intensive interaction between SDPP and implanted melatonin was also observed, the corresponding was 36.1%±0.79% for C&HN and 63.9%±0.79% for BN (P<0.01). Study showed that the hormones relative to nitrogen partitioning varied with different treatments. The level of blood melatonin was decreased with the increasing of photoperiod, implanted groups were higher than unimplanted groups. Hormones relative to protein increasing, such as PRL, IGF-I were increased with the length of photoperiod, implanted groups were lower than unimplanted groups. INS, which was related to the fat synthesization, was decreased with increasing of photoperiod, implanted groups were higher than unimplanted groups. LEP, which was related to the fat degeneration was increased with increasing of photoperiod, implanted groups were lower than unimplanted groups. As a result, average additive cashmere production was （338.83±72） g in SDPP and implanted groups, increased by 73.86%. Textile value of new cashmere was up to the standard.This study provided evidences that melatonin and photoperiod play an important role in nutrients partitioning in cashmere goats.

An in vitro procedure that simulated digestion in broilers was tested to evaluate the effects of graded level phytase adding in corn-soybean meal diet on broiler performance and phosphorus utilization. Phosphorus digestibility and amount of Inorganic Phosphorus (IP)release from 6 diets with and without graded level phytase were determined in vitro. Phosphorus digestibility and IP released were improved by adding phytase and go up with the increase of phytase level. Both of digestibility of phosphorus and IP released by in vitro digestion of low NPP diets with graded level phytase have high correlation with broiler daily gain, feed intake, phosphorus apparent digestibility in ileum, tibia ash , phosphorus in tibia and phosphorus in tibia ash of broiler (R²=0.971, 0.970; 0.990, 0.987; 0.962, 0.976; 0.903, 0.898; 0.866,0.859; 0.986, 0.984). So, we can get a conclusion that the in vitro digestion device for broiler can be used to evaluate and predict the effects of graded level phytase adding in broiler cornsoybean meal diets on broiler performance and phosphorus utilization.

Some visual information is sent to the nucleus geniculatus lateralis ventralis (GLv) via the cells in layer I (I cells) of the tectum in birds and is used for color vision, papillary reflex, and kineoptic functions. To reveal the morphological features of ‘I cells’ which project to GLv, they were retrogradely labeled with cyanine perchlorate DiI in chicks. Two different types of neurons, “spear dendritic I cells” and “forked dendritic I cells” were identified. The former had small spindle soma and an apical dendrite extending to the tectal surface, and the latter had somewhat larger triangular or polygonal soma and plural ascending dendrites. Most of the labeled dendritic endings distributed horizontally in layer F, and showed ending patterns similar to the terminals of optic nerve fibers.

The recombinant retroviral vector pBABE-puro-E2 was constructed by inserting E2 gene full-length cDNA of CSFV Shimen strain into pBABE-puro. Both the recombinant retroviral vector and pVSVg plasmid were transfected into 293T cells by calcium phosphate transfection method, thus the pseudovirus were produced. The pseudovirus infected eukaryotic cells SP2/0 and expression E2 protein was determined by puromycin-resistant and FACS analysis.BALB/c mice were injected in abdomen with expressing E2 protein SP2/0 cells. Anti-CSFV E2 antibody was screened by FACS. The results showed that CSFV E2 protein was expressed in SP2/0 cells’ envelope protein successfully. FACS could detect specific anti-E2 antibody in immune mouse serum. Mouse spleen cells were fused with myeloma SP2/0. Culture supernatants of hybridoms were screened by FACS and four cell lines which could secrete against E2 protein of CSFV McAbs stably were obtained. The McAbs not only react with CSFV specially but also had neutralization activity. The McAbs type belongs to IgM.

Three strains of infectious bronchitis virus were isolated from chicken farm in China, which were TJ9602 strain from Tianjin, HN9604 strain from Henan and BJ9601 strain from Beijing. Every virus strain were studied with chicken recurrent infection, biological characteristics assay, seroneutralization test, localization of IBV antigens, amplification of S1 gene and nucleic acid sequence analysis. The results showed that the three IBV strains were nephropathogenic strains and the target organs were the kidneys of chickens. HN9604 strain and BJ9601 strain shared highest homology of S1 gene sequence with H120 strain. HN9604, BJ9601, M41 and H120 can crossreact in seroneutralization test. In phylogenetic tree HN9604, BJ9601 were located in the cladogram embranchment A that includes H120 strain，they all belong to group Ⅰ. However, the TJ9602 strain is in another cladogram embranchment of group Ⅱ. It shared less homology of S1 gene sequence and shared weak crossreact in seroneutralization test with H120 strain or M41 strain.

The experiment was conducted to study the effect of copper toxicity on blood findings in chickens. 180 one-day-old Avian broilers were divided into three groups, and fed on diets as follow: （1）control (Cu 11.92 mg/kg)，（2）copper toxic I (Cu 650 mg/kg) and （3）copper toxic Ⅱ ( Cu 850 mg/kg) for six weeks. The signs began to appear at the 2nd week in copper toxic group Ⅱ and at the 3rd week in copper toxic group I. Some of affected chickens died during the experiment. Hematopathologically, red cells in toxic groups I and II became deformed. The populations of red cells, hemoglobin contents and serum caeruloplasmin activity were much lower, and the serum copper concentrations, activities of lactate dehydrogenase and glutamicoxaloacetic transaminase were much higher in copper toxic groups than in control group. Mechanism of copper toxicity on blood findings is also discussed .

The effects of compound butafosfan solution on chronic heat stress were studied by detecting the performance, body temperature, contents of some endocrine hormones （such as T₃, T₄, insulin and cortisol） and some serum biochemical parameters (such as CPK, ALP, LDH and GLU) of chronic heatstressed broilers respectively. 45 One-day-old broilers were randomly assigned to 3 treatment groups, 15 birds each, and were administered orally saline water and two levels of compound butafosfan solution. The birds were fed basal diet and reared further two weeks under high ambient temperature after heat exposure at 14 days old. The experimental results showed that the compound can improve the performance to a certain extent in heatstressed broilers, and also can significantly influence the serum endocrine hormones and biochemical parameters. The preparation can notably decrease the rectal temperature and serum contents of CPK, ALP and LDH, however, can increase the T₃/T₄ ratio and contents of insulin and glucose in chronic heat-stressed broilers. But the compound has no effect on serum cortisol content in heat-stressed broilers. Therefore, the compound butafosfan solution could exert the anti-stress action by regulating the contents of some endocrine hormones and altering some biochemical indices in heatstressed broilers.

The residue was extracted by hot methanol from Oxytropis glacialis powder, acidified with 1mol/L HCl and filtrated to get acid liquid. The acid liquid was extracted with chloroform to remove impurity. Then, its pH was adjusted to 9-10 with NaOH. The solution was extracted respectively with chloroform，ethyl acetate and n-butanol. The most alkaloids were maximum polarity in the butanol phase, with TLC checking on alkaloid，there were 10 alkaloids in chloroform phase, 7 alkaloids in ethyl acetate and 5 alkaloids in butanol phase. Applied the crude alkaloid extracted with butanol to a silica gel column, eluted with different proportion of ethyl acetate and methanol, collected 30-50 mL as one sample, monitored by TLC, incorporated the same fraction. The Ehrlich’s colorable fraction were sublimated and white needles crystal were obtained. The crystal was detected by TLC, which appeared the same dot shape, color and Rf as the standard swainsonine. The results preliminary determined that crystal was swainsonine. The chemical structures were identified as swainsonine by means of MP and IR, MS, ¹HNMR, ¹³CNMR.

Three hundred male chickens (55.5g±5.2g) were randomly divided into three groups. The group I and II take orally Qingliang electuary I and II (0.1% of ration) in water, and the other one is used as control. Then at the 7th, 21st, 35th and 49th day of age, all chickens in the three groups were weighted and 5 chickens from each group were randomly sampled and killed, 2 cm of intestinal segments from the duodenum, jejunum and ileum were collected for measuring the length of intestinal villus. The results showed that Qingliang electuary II is better than Qingliang electuary I in improving the length of intestinal villus. Compared with the control, the length of intestinal villus in the duodenum, jejunum and ileum in Qingliang electuary II group increased by 0.38mm (P<0.01) at 49th day, 0.36mm (P<0.01) at 21st day and 0.16mm (P<0.01) at 35th day respectively. The weight of chickens was continuously gained along with the length of intestinal villus. At 35 days old the weight in Qingliang electuary II increased by 79.44g (P<0.01) as compared with that in the control. The body weight is positive correlation with length of intestinal villus in chicken. It was hinted that the Qingliang electuary have obvious effects on lengthening intestinal villus and accelerating growth in chicken.

Mammalian β-defensins are endogenous cysteine-rich peptide antibiotics that are produced either by epithelial cells lining the respiratory, digestive and urogenital tracts or by granulocytes and macrophages. Here, we examined the expressive tissues of caBD-1 mRNA in camels through RNA in situ hybridization using the digoxigenin-labeled antisense nucleic acid probe which is 42 bp and was designed and synthesized according the known caBD-1 cDNA sequence.The result indicates that caBD-1 mRNA express in the stratified squamous epithelium of dorsal tongue and the columnar epithelium of intestinal crypts.

Gene frequency, polymorphism information contents, number of effective alleles and heterozygosity were analysed in Xiangdong black goat, Nanjiang yellow goat, Guizhou black goat, Bore goat by using microsatellite markers BMS2508.The results revealed polymorphism content information and number of effective alleles and heterozygosity for BMS2508 in those 4 goat breeds were 0.804 7,5.316 3,0.810 1； 0.689 4,4.404 9,0.700 6; 0.691 0,3.466 3,0.711 4; 0.768 8,4.444 9,0.775 0 respectively. It could be concluded, they were in genetic polymorphism at the microsatellite locus BMS2508. So microsatellite marker BMS2508 could be applied to genetic diversity evaluation in goat breeds . It was also a basis research on relationship between polymorphisms of the microsatellite locus and reproduction performance.

Primordial germ cells (PGCs) isolated from gonads of stage 19 by Ficoll density gradient centrifugation were cultured in DMEM medium in vitro. Then the second passage PGCs were induced to differentiate into neuron-like cells by different induction media. Specific markers and structures were detected by histochemistry method. The results showed that induction medium RA and IBMX were the best ways to induce PGCs into neuron-like cells, and almost 80%-85% cells exhibited typical neuron-like phenotype after induction. Special Nissl body was found by histochemistry. The effect of induction medium RA+IBMS,RA+BHA+DMSO and RA+BHA+DMSO+IBMX were better than that of induction medium BHA+DMSO.Under the same induction medium, about 80%-85% neuronlike cells could be detected no matter what PGCs were cultured 24 h, 48h or 72 h in vitro.

A microarray technique was developed by preparing highly conserved DNA fragments for five poultry respiratory tract diseases (Newcastle disease virus,avian influenza,infectious bronchitis virus, infectious laryngotracheitis virus, Mycoplasma gallisepticum)using molecular cloning methods and the DNA fragments were spotted onto NC filter to form microarrays. RNA extracted from samples was reversetranscripted, then followed by PCR amplification and labling with biotin-11-dUTP.The labeled DNA and prepared DNA microarray were subjected to specific hybridization, the hybridization results were scanned and analyzed with a scanner. The results showed that the microarray technique could identify and distinguish the five poultry respiratory tract diseases. The method is rapid, sensitive and specific, and can screen or quarantine a large number ample samples within a very short time.

Here we report the construction of sheep PrP gene standard plasmid DNA and curve using real-time RT-PCR. Total RNA was extracted from each sample and the fragments of target gene were amplified by RT-PCR. The plasmid was constructed for calibrating unknown samples. In this study, the constructed plasmid containing only the target gene was used to construct a calibration curve. The absolute standard curve method was shown to be of high linearity, sensitivity and reproducibility. The purpose of this study is to investigate the quantification of PrP mRNA expression for knowing the scrapie pathogenesis and providing powerful tool for further studies on prion diseases pathogenesis.

The experiments were carried out to investigate the optimum immune age and immune dosage of GC₀₂ coccidian vaccine. Ninety healthy cross broilers were chosen and randomly divided intoⅠ,Ⅱ,Ⅲ,Ⅳ,Ⅴ,Ⅵ groups(n=15) to study the immune effect of different immune dosage at the same immune times. Each chicken in Ⅰ-Ⅳ groups were orally inoculated with 4 000 ,8 000,16 000,20 000 sporulated oocysts of GC₀₂ coccidian vaccine, respectively . Chickens in Ⅴ group were not infected with sporulated oocysts and chickens in Ⅵ group were not inoculated. On the 7th day after the second immunity,Ⅰ-Ⅳgroups were orally challenged with 1.0×10⁵ sporulated oocysts for each chicken. On the 8th day after challenging with the sporulated oocysts, all chicken were slaughtered. According to lesion scoring and faces scoring, OPG, ACI, relative growth rate, feed conversion, etc., the effects of different immune dosage of GC₀₂ coccidian vaccine were evaluated. The results suggested that optimum immunization method was inoculating 8 000 sporulated oocysts per bird two times.Onehundred and eighty healthy chickens were randomly divided into A,B,C,D,E,F,G₁,G₂,G₃ groups (n=20) to study the immune effects of different immune age with the same immune dosage. Chickens in A,B,C groups were immunized with GC₀₂ coccidian vaccine at the different immune age; D,E,F and G₁,G₂,G₃ were positive and negative control groups respectively, the immune and challenge is the same as experiment one. The results concluded that GC₀₂ coccidian vaccine is safe and effectual in preventing coccidiosis, immunize with 8 000 sporalated oocysts per bird at the age of 7 days and 14 days can obtain the ideal effects.