This experiment was conducted to study the association of the polymorphism of partial Adipocyte Fatty AcidBinding Protein (AFABP) gene with the content of intramuscular fat and serum Triglyceride in ducks. A pair of primers Pin1 was designed according to the sequences of AFABP gene mRNA in duck and AFABP gene in chicken to amplify duck intron1 of AFABP gene, then 4 pairs of primers were designed according to the cloned gene. The SNPs were detected by PCRSSCP, confirmed by sequencing, then the association of polymorphism of AFABP gene with the content of intramuscular fat and serum Triglyceride was studied. The results indicated that the product by Pin1 included partial exon1, exon2 and complete intron1 of AFABP gene after clone and sequence, and homologous comparison was done between duck and chicken AFABP gene, which showed the homology was 75.1%. Three and six nucleotide mutations were found in the products by primer P1 and P3, respectively, which resulted in three and eight genotypes, respectively. The results of ChiSquare test showed that Cherry Valley duck and Sumu duck deviated from the HardyWeinberg equilibrium (P<0.01) except Jingding duck in P1 locus, and all of the three groups fit with it in P3 locus (P>0.05) , while the distribution of them was different in various groups (P<0.05 or P<0.01) at the same time. The least square analysis showed that the content of IMF and TG were significant difference in different groups (P<0.05), but the content of IMF had no significant difference between genotypes in P1 and P3 (P>0.05), only content of TG had significant difference in various genotypes in P3 and the ducks with CD genotype had higher content of TG than those with other genotypes (P<0.05). These results suggested that polymorphism in intron1 of AFABP gene have certain effect on TG content, also indicated that it needs further study to get a conclusion if these SNPs can influence the intramuscular fat trait in duck.

The aim of this study was to investigate the effect of manganese on the hepatic cytochrome P450 enzyme system in cocks. 400 fiftyday old Hyline brown cocks were randomly divided into four groups. The cocks in four groups were respectively fed with the basal diet containing 0, 600, 900 and 1 800 mg·kg1 MnCl2 to establish the subchronic manganism model. After 30, 60 and 90day treatment, the livers in every group were collected to detect the activity of microsomal cytochrome P450 enzyme system and the transcription level of CYP2H1 gene. The contents of cytochrome P450 and b5, the activities of aminopyrinNdemethylase(AND) and aniline4hydroxylase(AH) were gradually declined with the increase of manganese in diet. The high dose group was significantly lower than the control group while there were no difference between the low and middle dose group and the control group. There were no markedly changes in the activities of NADPHcytochrome C reductase(CR) and erythromycinNdemethylase(ERND). The activity of CR declined at 30 and 60 d, but increased at 90 d. Besides, the CR activity of the high dose group was significantly higher than that in the control group（P<0.01）. The activity of ERND increased at 30 d but no markedly tendency appeared at 60 and 90 d. The ERND activity in the high dose group was significantly higher than that in the low dose group（P<0.01）. The transcription level of CYP2H1 mRNA in the low dose and high dose group at 30 d, the middle dose group at 60 d, and the low dose group at 90 d were higher than that in the control group. The transcription level of CYP2H1 mRNA in other treatment groups were lower than that in the control group. These results indicated that manganism could significantly changes the activities of microsomalcytochrome P450s and the transcription level of CYP2H1 mRNA.

To determine the phenotypic and genotypic resistance of campylobacter coli(C.coli) isolates from poultry meat, 54 C.coli isolates from retail poultry meat were phenotyped for ciprofloxacin(CIP),erythromycin(ERY),doxycycline(DOX) and gentamicin(GEN) by agar dilution method, and were genotyped by multilocus sequence typing (MLST). Within the 54 C.coli isolates,resistance only to CIP,ERY and DOX were respectively 16.7% ,16.7% and 42.6%, 18.5% of the isolates were resistant to two or three antimicrobial agents,whereas none of the isolates were resistant to GEN.MLST analysis of 54 isolates identified 38 alleles and 26 STs, including 1 new allele and 8 new STs.26 STs revealed ST828 clonal complexes, ST1150 clonal complexes and 5 STs not assigned clonal complexes in the international databases at http://pubmlst.org/campylobacter/. ST828 clonal complexs were predominant(83.3%,45 isolates). MLST did not group the isolates by their resistance and susceptibility to these four antimicrobial agents. These results indicated that resistance to ciprofloxacin, erythromycin and doxycycline were found in C. coli from poultry meat, and ST828 clonal complex was mainly clonal complex in poultry meat. There was no linear relationships between the resistance and susceptibility to these four antimicrobial agent and ST clonal or ST clonal complexs.