TGF-β1及其受体在性成熟前后藏绵羊睾丸的表达比较

doi:10.11843/j.issn.0366-6964.2019.04.008

Abstract

Abstract:

This experiment was conducted to compare the distribution characteristics of TGF-β1 and its receptors(TGF-βRⅠ) in Tibetan sheep testis before and after sexual mature and attempt to explore their relationships with testicular spermatogenesis in sexual mature. The testis of 1.0-, 1.5-, 2.0-year-old Tibetan sheep were prepared by testicular removal surgery for describe the distribution density and the location of TGF-β1 and TGF-βRⅠ in the testis tissues using the Western blot, immunohistochemical SP combined with IPP (Image-Pro Plus) statistics methods. The results suggested that:1) The reactions of TGF-β1 antibody were weakly and there were no significant difference in Tibetan sheep testis before and after sexual mature(P>0.05);The expression of TGF-βR Ⅰ in the 1.5-year-old sheep testis tissue were significantly higher than 1.0-and 2.0-year-old(P<0.05). 2)The TGF-β1 was mainly present in Leydig cells and endothelial cells of small vascular at different ages, there were medium positive expression of TGF-β1 in Sertoli cells and spermatocytes at 1.5-year-old Tibetan sheep but weakly expressed in seminiferous epithelium at other 2 groups. The TGF-βRⅠwere expressed in seminiferous epithelium at 3 ages. In the 1.0-year-old sheep testis, the expression of TGF-βRⅠwas weakly expressed in Leydig cells, and at the age of 1.5, its expression in the round spermatid, Leydig cells and lymphatic endothelial cells were strongly positive, also there were immunolocalized in Leydig cells and capillary lymph vessel at 2-year-old, basically the expression of TGF-βRⅠin the 1.5-year-old sheep testis was more stronger than that of other 2 groups.3)The positive signal statistics indicated that the average absorbance of TGF-βRⅠ expression was significantly higher in the testis than TGF-β1,and the expression of TGF-β1 were not different in the testis of the Tibetan sheep before and after sexual mature(P> 0.05),the TGF-βRⅠwas expressed significantly higher in testis tissue of 1.5-year-old Tibetan sheep than that of 1.0-and 2.0-year-old sheep(P<0.05). In conclusion, these results demonstrate that the expression of TGF-β1 and TGF-βRⅠin Tibetan sheep testis appered with rise trend first and then fall with age in sexual mature, their expression in Sertoli cells had correlaton with the regulation of spermatogenesis; and the mainly changes in Leydig cells serve as a meaningful index for the further research of TGF-β1/TGF-βRⅠpathways involved in its steroid biosynthesis and basic insights into testicular development.

Key words: sexual mature, Tibetan sheep, testis, TGF-β1, receptor

CLC Number:

YUAN Ligang, SONG Xiao, YANG Jie, LIANG Xiaxia, CHEN Guojuan. Comparison of Distribution Characteristics of TGF-β1 and Its Receptors in the Testis of the Tibetan Sheep before and after Sexual Mature[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(4): 750-757.

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Comparison of Distribution Characteristics of TGF-β1 and Its Receptors in the Testis of the Tibetan Sheep before and after Sexual Mature

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