猪LYRM1基因对脂肪沉积的影响研究

doi:10.11843/j.issn.0366-6964.2019.04.001

Abstract

Abstract:

The purpose of this study was to explore the effect of pig LYRM1 gene on the fat deposition. The expression levels of LYRM1 gene in different tissues of Baixi pigs aged 10 months were first detected by qRT-PCR. CDS of LYRM1 gene was amplified by PCR. The recombinant eukaryotic expression vector pEGFP-N3-LYRM1 was constructed and then transfected into progenitor cells of subcutaneous adipose tissue of Baixi pig. The cell apoptosis of transfected cells was analyzed by triacylglycerol concentration detection, serum starvation method and MTT assay. The mRNA expression levels of LYRM1, PPARγ, ATGL and FAS genes were detected by qRT-PCR. The results showed that LYRM1 gene had the highest expression level in adipose tissues of Baixi pigs. The concentration of triacylglycerol in progenitor cells of subcutaneous adipose tissue transfected with pEGFP-N3-LYRM1 was higher than that in the control group. The apoptosis rates of the experimental group cells and the control group cells were similar and no significant difference was found. The mRNA expression levels of LYRM1, PPARγ, ATGL and FAS genes in the experimental group were significantly higher than that in the control group (P<0.01). In conclusion, overexpression of LYRM1 gene can increase the concentration of triacylglycerol, promote fat deposition, enhance the mRNA expression level of genes related to fat deposition, and indirectly increase the level of fat deposition. Therefore, LYRM1 gene can be regarded as a candidate gene to study the fat deposition, which provide a theoretical basis for investigating the mechanism of fat deposition.

Key words: LYRM1, triacylglycerol, apoptosis, fat deposition

CLC Number:

S828.2

YUAN Hongxia, LUO Jinhong, FENG Wenwu, CHEN Xiang. Study on the Effect of LYRM1 Gene on the Fat Deposition of Pig[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(4): 677-687.

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Study on the Effect of LYRM1 Gene on the Fat Deposition of Pig

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