ANXA2与ERp29在正常与临床型乳房炎绵羊乳腺组织中的表达与定位

doi:10.11843/j.issn.0366-6964.2018.06.024

Abstract

Abstract:

The study aimed to investigate the expression and localization of annexin2 (ANXA2) and endoplasmic reticulum protein 29 (ERp29) genes in mammary gland tissue of normal and clinical mastitis sheep (Ovis aries). In this study, mammary gland tissues of 3 normal and 3 clinical mastitis sheep (Ovis aries) were sampled. qRT-PCR, Western blot (WB) and immunohistochemistry (IHC) were used to detect the expression and distribution of ANXA2 and ERp29 mRNA and proteins in mammary gland tissue of normal and clinical mastitis sheep.The results showed that the expression of ANXA2 mRNA in the mammary gland tissue of clinical mastitis sheep was significantly higher than that of normal group (P<0.01), while the expression of ANXA2 protein was significantly lower than that of normal group (P<0.01). The expression of ERp29 mRNA and protein in the mammary gland tissue of clinical mastitis sheep were significantly higher than that of normal group (P<0.01). ANXA2 and ERp29 proteins had positive reactions in mammary gland tissues of normal and clinical mastitis sheep respectively and mainly located in mammary epithelial cells. The expression of ANXA2 and ERp29 were significantly different in the mammary gland tissue of normal and clinical mastitis sheep, which may be involved in the regulation of the occurrence and development of sheep mastitis.

Key words: sheep, mastitis, ANXA2, ERp29, expression, location

CLC Number:

S826.2

GAO Jian-feng, LI Tao-tao, CHEN Can-can, ZHAO Xing-xu, MA You-ji. Expression and Localization of ANXA2 and ERp29 in Mammary Gland Tissue of Normal and Clinical Mastitis Sheep(Ovis aries)[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(6): 1307-1313.

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Expression and Localization of ANXA2 and ERp29 in Mammary Gland Tissue of Normal and Clinical Mastitis Sheep(Ovis aries)

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