BMP15外显子1 SNPs检测及其与邵伯鸡母系产蛋性状的关联性分析

Abstract

Abstract: The objective of the present study were to elucidate the relationship between polymorphisms of bone morphogenetic protein 15 (BMP15) gene and egg production traits in female line of Shaobo chicken and to provide a scientific basis for marker-assisted selection for reproductive traits of chicken. Polymorphisms of exon1 of BMP15 gene were detected in 261 female line of Shaobo chicken by PCR-RFLP. The relationship between polymorphisms of BMP15 gene and egg production traits in female line of Shaobo chicken was analyzed by least squares linear model. Three polymorphic sites C397T, A474G and C594T were found in exon1 of BMP15, and C397T mutation resulted in an amino acid change of leucine→phenylalanine. Three genotypes were found at all three mutation sites in 261 female line of Shaobo chicken by RFLP. Chi-square test showed that Shaobo chickens were in Hardy-Weinberg equilibrium. The relationship between three site polymorphisms of BMP15 gene and egg production traits in Shaobo chickens was analyzed by least squares linear model. The result showed that three genotypes (CC, CT and TT) were found at C397T mutation locus, and individuals with TT genotype had significant younger age at first egg than that for CT (P<0.05), TT genotype birds had also significant more the total number of eggs at the age of 300 days than that for CT (P<0.05). Three genotypes (AA, AG and GG) were found at A474G mutation site, and there was no significant effect on egg production traits at the locus. Three genotypes (CC, CT and TT) were found at C594T mutation locus, and CC genotype had significant younger age at first egg than that for CT and TT (P<0.05). The combined genotype TTAATT also had significant genetic effects on age at first egg (AFE), weight at first egg(WFE), body weight at first egg(BWFE) and the total number of eggs with 300 age(EN)（P<0.05）. The combined genotype TTAATT was the most favorable genotype. The result indicate that combined genotype TTAATT of BMP15 gene is a potential DNA marker for improving egg production traits in female line of Shaobo chicken.

Key words: Shaobo chicken, bone morphogenetic protein 15 gene, egg production traits

CLC Number:

S831.2

LI Chun-miao, LI Shou-feng, ZHAO Zhen-hua, HUANG Hua-yun, XUE Long-gang. Detection of SNPs of Bone Morphogenetic Protein 15 Gene Exon1 and Its Association with Egg Production Traits in Female Line of Shaobo Chicken[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA.

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Detection of SNPs of Bone Morphogenetic Protein 15 Gene Exon1 and Its Association with Egg Production Traits in Female Line of Shaobo Chicken

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