过表达IL-6对山羊毛囊干细胞增殖和迁移的影响

doi:10.11843/j.issn.0366-6964.2019.06.005

Abstract

Abstract: The aim of this study was to investigate the effects of overexpressing inflammatory factor interleukin-6 (IL-6) on the proliferation and migration of goat hair follicle stem cells (HFSCs) and elucidate the role of MAPK/ERK signaling pathway in the proliferation and migration of goat HFSCs. The pXJ40-myc-IL-6 overexpression vector was constructed and transiently transfected into the goat HFSCs, and the empty vector cells were used as the blank control group. Western blot was used to quantitatively detect the expression of IL-6 protein, and MTT assay was used to detect the proliferation of goat HFSCs. The migration ability of HFSCs was detected by wound healing assay. Finally, Western blot was used to detect the expression of key kinases in MAPK/ERK and P38 signaling pathway in goat HFSCs. The results showed that after transfecting the IL-6 overexpression vector into goat HFSCs for 3 days, compared with the control group, the proliferation activity of the HFSCs was inhibited and the sustained inhibition effect was observed until the 7th day(P<0.05). After 24 h of transfection of IL-6 overexpression vector into goat HFSCs, the healing rate of wounding site in the treatment group reached 84.2%, which was significantly lower than that in the control group (98.5%, P<0.01). Compared with the control group, the phosphorylation level of the key kinase ERK1/2 (p-ERK1/2) in the MAPK/ERK signaling pathway was significantly down-regulated (P<0.01), the phosphorylation level of the key kinase p38 (p-p38) in the P38 signaling pathway was up-regulated, however, the change was not statistically significant (P>0.05). In summary, the overexpression of IL-6 gene has an inhibitory effect on the proliferation and migration ability of goat HFSCs, and its negative regulatory role on the proliferation and migration of goat HFSCs maybe through the MAPK/REK signaling pathway. The results provide basis for revealing the mechanism of migration and tissue repair of goat HFSCs.

CLC Number:

Q813

SHI Mingyan, GAO Xue, YI Li, ZHOU Xinyu, KANG Junfang, LI Zhichao. Effects of Overexpression of IL-6 on Proliferation and Migration of Goat Hair Follicle Stem Cells in vitro[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(6): 1154-1161.

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Effects of Overexpression of IL-6 on Proliferation and Migration of Goat Hair Follicle Stem Cells in vitro

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